JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248682

Anti-LSP1 antibody [EPR3160] - BSA and Azide free

Be the first to review this product! 리뷰 제출

|

(1 출판물)

Rabbit Recombinant Monoclonal LSP1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

WP34, LSP1, Lymphocyte-specific protein 1, 47 kDa actin-binding protein, 52 kDa phosphoprotein, Lymphocyte-specific antigen WP34, pp52

1 이미지
Western blot - Anti-LSP1 antibody [EPR3160] - BSA and Azide free (AB248682)
  • WB

Unknown

Western blot - Anti-LSP1 antibody [EPR3160] - BSA and Azide free (AB248682)

This data was developed using ab134099, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-LSP1 antibody [EPR3160] - BSA and Azide free (ab248682) at 1/1000 dilution

Lane 1:

Daudi cell lysate at 10 µg

Lane 2:

Ramos cell lysate at 10 µg

Lane 3:

HuT-7 cell lysate at 10 µg

Lane 4:

U937 cell lysate at 10 µg

Lane 5:

K562 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3160

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Primary Antibodies

AB214488

Anti-TROP2 antibody [EPR20043]

20ul selling size|BOND RX™ Validated|KO Validated|RabMAb|Recombinant

Western blot - Anti-TROP2 antibody [EPR20043] (AB214488)

  • 5
  • 9
Primary Antibodies

AB204583

Anti-Keratinocyte differentiation associated protein antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Keratinocyte differentiation associated protein antibody (AB204583)

  • 0
  • 0
Primary Antibodies

AB28081

Anti-MUC1 antibody [C595 (NCRC48)]

Immunocytochemistry/ Immunofluorescence - Anti-MUC1 antibody [C595 (NCRC48)] (AB28081)

  • 0
  • 0
Primary Antibodies

AB179475

Anti-Integrin alpha V antibody [EPR16800]

20ul selling size|KO Validated|RabMAb|Recombinant

Western blot - Anti-Integrin alpha V antibody [EPR16800] (AB179475)

  • 5
  • 10
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

ab248682 is the carrier-free version of ab134099.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Lymphocyte-specific protein 1 also known as LSP1 is a 52 kDa intracellular F-actin binding protein. It plays a role in cytoskeletal organization and cell motility. This protein has significant expression in lymphocytes neutrophils macrophages and endothelial cells. LSP1 modulates several cellular functions including adhesion and migration by interacting with the actin cytoskeleton. Known alternatively as pp52 and WD-40 repeat-containing protein 1 LSP1 acts as a link connecting signal transduction from cell surface receptors to cytoskeletal regions.
Biological function summary

LSP1 influences immune cell regulation and inflammatory response. By acting as an important player in the modulation of actin polymerization it contributes to leukocyte chemotaxis and transmigration. LSP1 forms part of a larger protein complex involved in reorganizing the cytoskeleton facilitating cellular dynamics necessary for immune response. Additionally interactions with adapter proteins like 14-3-3 add another level of functional regulation.

Pathways

LSP1 integrates into the chemokine signaling pathway and the P38 MAPK signaling pathway. In these pathways it exerts influence on cellular processes such as migration and response to external stimuli. It shows connections with proteins such as CXCR4 and MAPK highlighting its role in conveying chemotactic signals that direct immune cells toward inflammatory sites. These interactions are integral in establishing directionality and speed of cell movement in response to chemokine gradients.

Aberrations in LSP1 function or expression link to autoimmune conditions like systemic lupus erythematosus and vascular disorders like atherosclerosis. Its role in mediating vascular permeability and leukocyte migration suggests that dysregulation can contribute to pathogenic inflammation. Related proteins like MCP-1 and ICAM-1 participate in these disease pathways highlighting the involvement of LSP1 in modulating endothelial responses and immune cell recruitment at sites of inflammation.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

May play a role in mediating neutrophil activation and chemotaxis.
See full target information LSP1
style
left-column

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Theranostics 12:4513-4535 PubMed35832075

2022

Blockade of phosphotyrosine pathways suggesting SH2 superbinder as a novel therapy for pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,An-Dong Liu,Qian Niu,Xiao Feng,Yuan-Yi Zheng,Shuai-Jun Chen,Hui Xu,Qian Li,Guo-Qing Hou,Xiao-Yang Bi,Yu-Zhi Lu,Pei-Pei Cheng,Li-Mei Liang,Ye-Han Jiang,Li-Qin Zhao,Fei Liu,Lin-Jie Song,Li-Ling Zhou,Ling-Yan Xiao,Feng Chen,Shawn Shun-Cheng Li,Wan-Li Ma,Xuan Cao,Hong Ye
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com