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AB255804

Anti-Ly-6A/E (Sca-1) antibody [EPR22502-68] - BSA and Azide free

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Rabbit Recombinant Monoclonal Sca1 / Ly6A/E antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 1 publication.
3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR22502-68] - BSA and Azide free (AB255804)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR22502-68] - BSA and Azide free (AB255804)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Ly-6A/E (Sca1) with ab255604 at 1/1000 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on blood vessels of mouse cardiac muscle (PMID : 2660142) is observed. The section was incubated with ab255604 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255604).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR22502-68] - BSA and Azide free (AB255804)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR22502-68] - BSA and Azide free (AB255804)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Ly-6A/E (Sca1) with ab255604 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse kidney (PMID : 2660142) is observed. The section was incubated with ab255604 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255604).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR22502-68] - BSA and Azide free (AB255804)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR22502-68] - BSA and Azide free (AB255804)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Ly-6A/E (Sca1) with ab255604 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis (PMID : 2660142) is observed. The section was incubated with ab255604 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255604).

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22502-68

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB268016

FITC Anti-Ly-6A/E (Sca-1) antibody [D7]

Flow Cytometry - FITC Anti-Ly-6A/E (Sca-1) antibody [D7] (AB268016)

  • 0
  • 0
Primary Antibodies

AB51317

Anti-Ly-6A/E (Sca-1) antibody [E13 161-7] - Hematopoietic Stem Cell Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Ly-6A/E (Sca-1) antibody [E13 161-7] - Hematopoietic Stem Cell Marker (AB51317)

  • 5
  • 5
Primary Antibodies

AB239951

Anti-Ly-6A/E (Sca-1) antibody [EPR3355] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] - BSA and Azide free (AB239951)

  • 0
  • 0
Primary Antibodies

AB109211

Anti-Ly-6A/E (Sca-1) antibody [EPR3355]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ly-6A/E (Sca-1) antibody [EPR3355] (AB109211)

  • 4
  • 1

보완 제품

Sample Prep & Detection Kits

AB102918

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®

Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

  • 5
  • 2
Sample Prep & Detection Kits

AB269893

Latex Conjugation Kit - 400nm Red

Functional Studies - Latex Conjugation Kit - 400nm Red (AB269893)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB203491

Anti-PDGFR alpha antibody [EPR22059-270]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (AB203491)

  • 4
  • 7
Primary Antibodies

AB133357

Anti-CD11b antibody [EPR1344]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD11b antibody [EPR1344] (AB133357)

  • 4
  • 37
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

ab255804 is the carrier-free version of ab255604.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

T-cell activation.
See full target information Ly6a

대체 명칭 보기

Ly6, Ly6a, Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, T-cell-activating protein, SCA-1, TAP
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Proceedings of the National Academy of Sciences of the United States of America 122:e2514837122 PubMed40811463

2025

Stress granule-mediated ZBP1 activation drives necroptotic cell death in non-obstructive azoospermia and testicular aging.

Applications

Unspecified application

Species

Unspecified reactive species

Hongen Lei,Dianrong Li,Jie Chen,Baowen Du,Kaiju Jiang,Tao Xu,Hu Han,Weiliang Fan,Long Tian,Xiaodong Wang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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