AB126379

Anti-LYRM5 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal LYRM5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ETFRF1 aa 1 to C-terminus.

대체 명칭 보기

LYRM5, ETFRF1, Electron transfer flavoprotein regulatory factor 1, LYR motif-containing protein 5

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM5 antibody (AB126379)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM5 antibody (AB126379)

Immunohistochemical analysis of human stomach tissue labeling LYRM5 in the cytoplasm of glandular cells with ab126379 at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-LYRM5 antibody (AB126379)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-LYRM5 antibody (AB126379)

Immunocytochemical analysis of RH-30 (Human bone marrow metastasis cell line) whole cells labeling LYRM5 in the microtubule organizing center with ab126379 at 2 μg/ml.

Supplier Data

Western blot - Anti-LYRM5 antibody (AB126379)

All lanes:

Western blot - Anti-LYRM5 antibody (ab126379) at 1/100 dilution

Lane 1:

Negative control (vector only transfected) - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

LYRM5 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)

Predicted band size: 11 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ETFRF1 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6IPR1

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The LYRM5 protein also known as LYR motif containing 5 is a small protein with a mass of approximately 7 kDa. Researchers have identified its expression predominantly in mitochondrial regions where it plays a critical role in mitochondrial function. This protein forms part of the LYR family which is involved in various cellular activities. LYRM5's location in mitochondria suggests its direct involvement in mitochondrial processes and homeostasis.

Biological function summary

LYRM5 functions as an important component in mitochondrial bioenergetics. It associates with the complex known as NADH:ubiquinone oxidoreductase (Complex I) of the mitochondrial respiratory chain. Through this association LYRM5 supports the assembly and stabilization of Complex I a critical step in ATP production. This function highlights its importance in cellular energy metabolism emphasizing LYRM5’s role in maintaining proper mitochondrial function.

Pathways

LYRM5 is actively engaged in the oxidative phosphorylation pathway which is important for ATP generation in cells. It facilitates interactions within Complex I ensuring the efficient transfer of electrons in the electron transport chain. Additionally LYRM5 interacts with proteins such as NDUFS6 (part of Complex I) further illustrating its role in this vital energy-producing pathway.

Alterations or malfunctions of LYRM5 have been implicated in mitochondrial diseases often characterized by defects in energy production. Specifically studies link mutations in LYRM5 to disorders like Leigh syndrome a severe neurological condition. The protein's interaction with Complex I proteins like NDUFS6 also associates it with these mitochondrial pathologies suggesting a critical disease-related role for LYRM5 in maintaining mitochondrial integrity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a regulator of the electron transfer flavoprotein by promoting the removal of flavin from the ETF holoenzyme (composed of ETFA and ETFB).

See full target information ETFRF1

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Science advances 11:eadp4765 PubMed39841844

2025

WWC proteins-mediated compensatory mechanism restricts schwannomatosis driven by loss of function.

Applications

Unspecified application

Species

Unspecified reactive species

Xueying Wang,Rui Zhu,Pengcheng Yu,Sixian Qi,Zhenxing Zhong,Ruxin Jin,Yu Wang,Yuan Gu,Dan Ye,Kang Chen,Yilai Shu,Yi Wang,Fa-Xing Yu

Translational cancer research 11:519-529 PubMed35402183

2022

CircPTN promotes angiogenesis via the MiR-595/LYRM5 signaling pathway in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shengcong Guo,Zejun Mao,Guodong Zhang,Botao Xu,Xiangfeng He

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-LYRM5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LYRM5 antibody (AB126379)

Immunocytochemistry/ Immunofluorescence - Anti-LYRM5 antibody (AB126379)

Western blot - Anti-LYRM5 antibody (AB126379)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise