Anti-Lysozyme antibody [SP329]

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Lysozyme antibody [SP329] (AB223503)

Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cell line labeling Lysozyme with ab223503 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (AB223503)

Formalin-fixed, paraffin-embedded human tonsil tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (AB223503)

Formalin-fixed, paraffin-embedded human spleen tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (AB223503)

Formalin-fixed, paraffin-embedded human lung tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (AB223503)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 μg/ml). Cytoplasmic staining on the human spleen, performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab223503 for 10 mins at room temperature. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (AB223503)

Formalin-fixed, paraffin-embedded human thymus tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (AB223503)

Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (AB223503)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human stomach carcinoma tissue sections labeling Lysozyme with ab223503 at 1/100 dilution (2.62 μg/ml). Cytoplasmic staining on the human stomach carcinoma and the stromal cells, performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab223503 for 10 mins at room temperature. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (AB223503)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Lysozyme antibody [SP329] (AB223503)

Formalin-fixed, paraffin-embedded human liver tissue stained for Lysozyme using ab223503 at 1/100 dilution in immunohistochemical analysis.

• WB

Lab

Western blot - Anti-Lysozyme antibody [SP329] (AB223503)

Lanes 1 - 4 : Merged signal (red and green). Green - ab223503 observed at 16 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab223503 was shown to react with Lysozyme in wild-type THP1 cells in Western blot with loss of signal observed in LYZ knockout sample.Wild-type THP1 and LYZ knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween^®) before incubation with ab223503 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 400 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Lysozyme antibody [SP329] (ab223503) at 1/400 dilution

Lane 1:

Wild-type THP1 cell lysate at 20 µg

Lane 2:

LYZ knockout THP1 cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Predicted band size: 16 kDa

Observed band size: 16 kDa

false

• WB

Supplier Data

Western blot - Anti-Lysozyme antibody [SP329] (AB223503)

All lanes:

Western blot - Anti-Lysozyme antibody [SP329] (ab223503) at 1/400 dilution

All lanes:

HL-60 (human promyelocytic leukemia cell line) cell lysate

Predicted band size: 16 kDa

false