Anti-Mad2L1 antibody [17D10]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L1 antibody [17D10] (AB10691)

ab10691 staining Mad2L1 in colon cancer tissue, after antigen retreival, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

• WB

Lab

Western blot - Anti-Mad2L1 antibody [17D10] (AB10691)

Lanes 1 - 4 : Merged signal (red and green). Green - ab10691 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.

ab10691 was shown to specifically react with in wild-type HAP1 cells as signal was lost in MAD2L1 knockout cells. Wild-type and MAD2L1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab10691 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 2 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Mad2L1 antibody [17D10] (ab10691) at 2 µg/mL

Lane 1:

Wild-type HAP1 whole cell lysate at 20 µg

Lane 2:

Mad2L1 knockout HAP1 whole cell lysate at 20 µg

Lane 3:

HeLa whole cell lysate at 20 µg

Lane 4:

A431 whole cell lysate at 20 µg

Predicted band size: 24 kDa

Observed band size: 24 kDa

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• WB

AbReview20235****

Western blot - Anti-Mad2L1 antibody [17D10] (AB10691)

Mad2L1 antibody (ab10691) at 1/1000 (in TBS for 90 minutes at 22°C) + whole tissue lysate of Human liver biopsy (chronic hepatitis C - (HepC) or non-viral (NVH)) patients at 50μg.

Secondary Antibody : An HRP-conjugated Goat anti-mouse polyclonal (1/100000).

Blocking Step : 5% Milk for 16 hours at 4°C

All lanes:

Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

This image is courtesy of an Abreview submitted by Saira Sarfraz

• WB

Unknown

Western blot - Anti-Mad2L1 antibody [17D10] (AB10691)

Western blot using ab10691 at 1/500.

Lane 1 : Hela Nuclear
Lane 2 : Hela
Lane 3 : A431
Lane 4 : Jurkat
Lane 5 : 293

Mad2L1 predicted size : 24.6kD.

Secondary ab : Rabbit polyclonal to Mouse IgG H&L (HRP) ab6728 (1/5000)
Exposure time : 1min
20μg of lysate per lane.

Western blot using ab10691 at 1/500. Lane 1 : Hela Nuclear Lane 2 : Hela Lane 3 : A431 Lane 4 : Jurkat Lane 5 : 293 Mad2L1 predicted size : 24.6kD. Secondary ab : anti-mouse HRP (ab6728) (1/5000) Exposure time : 1min 20μg of lysate per lane.

All lanes:

Western blot - Anti-Mad2L1 antibody [17D10] (ab10691)

Predicted band size: 24 kDa

false