JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB209667

Anti-MAGEC2 antibody [EPR19064]

Be the first to review this product! 리뷰 제출

|

(5 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal MAGEC2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Recombinant full length protein samples. Cited in 5 publications.

대체 명칭 보기

HCA587, MAGEE1, MAGEC2, Melanoma-associated antigen C2, Cancer/testis antigen 10, Hepatocellular carcinoma-associated antigen 587, MAGE-C2 antigen, MAGE-E1 antigen, CT10

9 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (AB209667)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (AB209667)

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human gastric cancer is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-MAGEC2 antibody [EPR19064] (AB209667)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MAGEC2 antibody [EPR19064] (AB209667)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed A-375 (Human malignant melanoma cell line) cells labeling MAGEC2 with ab209667 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (AB209667)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (AB209667)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human germ cells is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MAGEC2 antibody [EPR19064] (AB209667)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAGEC2 antibody [EPR19064] (AB209667)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-375 (Human malignant melanoma cell line) and HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling MAGEC2 with ab209667 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing nuclear and weakly cytoplasmic staining on A-375 cell line

Negative expression in HeLa cells.

The nuclear counter stain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows :
-ve control 1 : ab209667 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (AB209667)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (AB209667)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human stomach. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (AB209667)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEC2 antibody [EPR19064] (AB209667)

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling MAGEC2 with ab209667 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on melanoma is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-MAGEC2 antibody [EPR19064] (AB209667)
  • IP

Supplier Data

Immunoprecipitation - Anti-MAGEC2 antibody [EPR19064] (AB209667)

MAGEC2 was immunoprecipitated from 1mg of A-375 (Human malignant melanoma cell line) whole cell lysate with ab209667 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab209667 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : A-375 whole cell lysate 10μg (Input).

Lane 2 : ab209667 IP in A-375 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab209667 in A-375 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-MAGEC2 antibody [EPR19064] (ab209667)

Predicted band size: 41 kDa

false

Western blot - Anti-MAGEC2 antibody [EPR19064] (AB209667)
  • WB

Supplier Data

Western blot - Anti-MAGEC2 antibody [EPR19064] (AB209667)

Blocking/Dilution buffer : 5% NFDM/TBST.

The band is consistent with what is observed in the literature (PMID : 16594646).

All lanes:

Western blot - Anti-MAGEC2 antibody [EPR19064] (ab209667) at 1/10000 dilution

Lane 1:

A-375 (Human malignant melanoma cell line) whole cell lysate at 10 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

Lane 4:

Mouse testis lysate at 10 µg

Lane 5:

Human fetal kidney lysate at 10 µg

Lane 6:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 41 kDa

Observed band size: 51 kDa

false

Exposure time: 30s

Western blot - Anti-MAGEC2 antibody [EPR19064] (AB209667)
  • WB

Supplier Data

Western blot - Anti-MAGEC2 antibody [EPR19064] (AB209667)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-MAGEC2 antibody [EPR19064] (ab209667) at 1/5000 dilution

All lanes:

Full length recombinant protein MAGEC2 at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 41 kDa

Observed band size: 51 kDa

false

Exposure time: 1s

관련 conjugated 항체와 다양한 조성의 항체 (1)

  • Carrier free

    Anti-MAGEC2 antibody [EPR19064] - BSA and Azide free

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19064

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

보완 제품

Primary Antibodies

AB219650

Anti-PRAME antibody [EPR20330]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRAME antibody [EPR20330] (AB219650)

  • 0
  • 0
Primary Antibodies

AB139297

Anti-MAGEA4 antibody [OTI1F9]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA4 antibody [OTI1F9] (AB139297)

  • 0
  • 0
Primary Antibodies

AB223498

Anti-CTAG1B antibody [SP349]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CTAG1B antibody [SP349] (AB223498)

  • 0
  • 0
Primary Antibodies

AB243570

Anti-SPERT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SPERT antibody (AB243570)

  • 4
  • 1
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "<p><a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Recombinant full length protein": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MAGEC2 also known as melanoma-associated antigen C2 or CT10 is a protein with a molecular mass of approximately 60 kDa. Researchers have found it expressed mostly in human cancerous tissues particularly in melanoma but not in normal tissues except for the testis. This restricted expression pattern has made MAGEC2 an interesting target for cancer immunotherapy and diagnosis. Its presence varies between different cancer types influencing its potential as a biomarker or therapeutic target.
Biological function summary

MAGEC2 interacts with cellular mechanisms related to cell growth and proliferation. The protein plays a role in processes linked to cancer progression by modulating transcriptional activities through its interaction with other proteins. MAGEC2 is not known to be part of a specific protein complex but its function associates with the ubiquitination process and modulation of p53 a well-known tumor suppressor protein. These interactions suggest critical roles in tumor maintenance and survival.

Pathways

MAGEC2's influence extends to oncogenic pathways involving cell cycle regulation and DNA damage response. It plays a part in the p53 signaling pathway where it impacts p53 stability and activity emphasizing its importance in cancer biology. Additionally connections with the E2F transcription factor highlight its potential role in regulating a series of genes important for cell cycle progression suggesting a strong interplay with cellular division processes.

The aberrant expression of MAGEC2 relates most closely to melanomas and carcinomas which demonstrate increased levels of this protein. The association with these cancers emphasizes its potential as a target for cancer immunotherapy. In melanomas MAGEC2 often implicates with the dysregulation of the p53 protein contributing to unchecked cell division and tumor growth. Other cancer types may show similar associations highlighting the universality of MAGEC2's role in tumorigenic processes.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. In vitro enhances ubiquitin ligase activity of TRIM28 and stimulates p53/TP53 ubiquitination in presence of Ubl-conjugating enzyme UBE2H leading to p53/TP53 degradation. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzymes (E2) at the E3 : substrate complex.
See full target information MAGEC2
style
left-column

제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell reports methods 4:100906 PubMed39561714

2024

Tumor-associated antigen prediction using a single-sample gene expression state inference algorithm.

Applications

Unspecified application

Species

Unspecified reactive species

Xinpei Yi,Hongwei Zhao,Shunjie Hu,Liangqing Dong,Yongchao Dou,Jing Li,Qiang Gao,Bing Zhang

Scientific reports 14:12546 PubMed38822068

2024

Pulsed electric field induces exocytosis and overexpression of MAGE antigens in melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wojciech Szlasa,Natalia Sauer,Dagmara Baczyńska,Marcin Ziętek,Katarzyna Haczkiewicz-Leśniak,Paweł Karpiński,Mariusz Fleszar,Paulina Fortuna,Michał J Kulus,Aleksandra Piotrowska,Alicja Kmiecik,Agnieszka Barańska,Olga Michel,Vitalij Novickij,Mounir Tarek,Paulina Kasperkiewicz,Piotr Dzięgiel,Marzenna Podhorska-Okołów,Jolanta Saczko,Julita Kulbacka

Journal of breast cancer 24:138-152 PubMed33818016

2021

Increased Melanoma-Associated Antigen C2 Expression Affords Resistance to Apoptotic Deathin Suspension-Cultured Tumor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Doyeon Park,Sora Han,Hyunjeong Joo,Hye In Ka,Sujung Soh,Jiyoung Park,Young Yang

Technology in cancer research & treatment 17:1533033818797587 PubMed30198403

2018

Study on Attenuating Angiogenesis and Epithelial-Mesenchymal Transition (EMT) of Non-Small Cell Lung Carcinoma (NSCLC) by Regulating MAGEC2.

Applications

Unspecified application

Species

Unspecified reactive species

Sicong Jiang,Xi Liu,Daojing Li,Meiying Yan,Cheng Ju,Jun Sun,Feng Jiang

Oncology letters 13:1609-1618 PubMed28454298

2017

Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer.

Applications

IHC-P

Species

Human

Xinfeng Chen,Liping Wang,Jinyan Liu,Lan Huang,Li Yang,Qun Gao,Xiaojuan Shi,Jieyao Li,Feng Li,Zhen Zhang,Song Zhao,Bin Zhang,Pierre Van der Bruggen,Yi Zhang
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com