AB184157

Anti-MAGMAS antibody [EPR15214]

RabMAb
Recombinant
각 태그의 뜻

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal MAGMAS antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications.

대체 명칭 보기

MAGMAS, TIM16, TIMM16, CGI-136, PAM16, Mitochondrial import inner membrane translocase subunit TIM16, Mitochondria-associated granulocyte macrophage CSF-signaling molecule, Presequence translocated-associated motor subunit PAM16

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-MAGMAS antibody [EPR15214] (AB184157)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAGMAS antibody [EPR15214] (AB184157)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling MAGMAS with ab184157 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with DAPI (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] (AB184157)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] (AB184157)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling MAGMAS with ab184157 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] (AB184157)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling MAGMAS with ab184157 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained using hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-MAGMAS antibody [EPR15214] (AB184157)

All lanes:

Western blot - Anti-MAGMAS antibody [EPR15214] (ab184157) at 1/20000 dilution

All lanes:

Human skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 13 kDa

false

Supplier Data

Western blot - Anti-MAGMAS antibody [EPR15214] (AB184157)

All lanes:

Western blot - Anti-MAGMAS antibody [EPR15214] (ab184157) at 1/20000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

LnCaP cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 13 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15214

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Regulates ATP-dependent protein translocation into the mitochondrial matrix. Inhibits DNAJC19 stimulation of HSPA9/Mortalin ATPase activity.

See full target information PAM16

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nutrients 16: PubMed39339680

2024

Combined Aerobic Exercise Training and Intake Reduces Arterial Stiffness through Enhanced Arterial Nitric Oxide Production in Obese Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Henry Yamazaki,Shumpei Fujie,Kenichiro Inoue,Masataka Uchida,Motoyuki Iemitsu

International journal of molecular sciences 21: PubMed33167377

2020

Insight into the Interactome of Intramitochondrial PKA Using Biotinylation-Proximity Labeling.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmine Ould Amer,Etienne Hebert-Chatelain

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAGMAS antibody [EPR15214]

Immunocytochemistry/ Immunofluorescence - Anti-MAGMAS antibody [EPR15214] (AB184157)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] (AB184157)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGMAS antibody [EPR15214] (AB184157)

Western blot - Anti-MAGMAS antibody [EPR15214] (AB184157)

Western blot - Anti-MAGMAS antibody [EPR15214] (AB184157)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise