AB243066

Anti-Malondialdehyde antibody [11E3]

Anti-Malondialdehyde 항체 [11E3]

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(25 제품이 사용된 논문 )

Anti-Malondialdehyde antibody [11E3] (ab243066) is a mouse monoclonal antibody detecting Malondialdehyde in Western Blot, IHC-P.

대체 명칭 보기

1133 Tetramethoxypropane

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Malondialdehyde antibody [11E3] (AB243066)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Malondialdehyde antibody [11E3] (AB243066)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Malondialdehyde with ab243066 (green). Tissue was incubated with ab243066 at 1/100 overnight at 4^oC then for 30 minutes at 37^oC, followed by incubation with a Goat anti-Mouse IgG (H+L) : FITC secondary antibody for 45 minutes at 37^oC. Counterstain used was DAPI for 3 minutes at RT. Magnification : 5X.

Supplier Data

Western blot - Anti-Malondialdehyde antibody [11E3] (AB243066)

Primary incubation for 2 hours at room temperature.

Secondary incubation for 1 hour at room temperature.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-Malondialdehyde antibody [11E3] (ab243066) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 12 µg

Lane 2:

HeLa (treated with hydrogen peroxide) cell lysate at 12 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

true

Exposure time: 5min

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

11E3

Isotype

IgG1

Carrier free

Applications

IHC-P, WB

applications

Specificity

Specific for MDA conjugated proteins. Does not detect free MDA. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-HHE, 4-HNE, or Methylglyoxal modified proteins.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-Malondialdehyde antibody [11E3] (ab243066) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P).

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from TCS.

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Malondialdehyde often abbreviated as MDA is a reactive organic compound formed by the degradation of polyunsaturated lipids. This breakdown occurs through lipid peroxidation which results in MDA being a well-recognized marker for oxidative stress. The molecular mass of malondialdehyde is relatively low around 72.06 g/mol. This small molecule does not localize to specific tissues but instead it can be found throughout the body wherever lipid peroxidation occurs making it widespread in biological systems.

Biological function summary

This compound plays an important role in signaling processes related to oxidative stress. MDA is not part of a protein complex but it interacts with proteins by forming adducts with lysine and histidine residues. Such adduct formations may alter protein function and structure providing insights into oxidative modifications of biomolecules. In research settings scientists often use malondialdehyde tests such as the malondialdehyde ELISA kit to quantify MDA levels as a sign of oxidative damage in cells and tissues.

Pathways

Malondialdehyde integrates into the lipid peroxidation pathway. This pathway functions as a feedback loop indicating oxidative stress conditions within cells. Another related pathway includes the arachidonic acid metabolism. MDA formation signals further lipid peroxidation impacting the interaction with several proteins including cyclooxygenases which are involved in inflammation processes.

Malondialdehyde serves as a biomarker for conditions associated with oxidative stress such as cardiovascular diseases and neurodegenerative disorders. Studies have linked elevated MDA levels with cardiovascular diseases where it can interact with low-density lipoproteins marking them for uptake by macrophages. Similarly in neurodegenerative disorders like Alzheimer's malondialdehyde's levels correlate with protein dysfunctions. These are also often observed in conjunction with beta-amyloid interactions contributing to disease pathology. Researchers frequently employ MDA to KDAs in detailed analyses to understand these processes better.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Malondialdehyde

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제품이 사용된 논문 (25)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in pharmacology 16:1617185 PubMed40926987

2025

Renoprotective effects of GHRH agonist MR409 is associated with reduced oxidative stress and ferroptosis in diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yueyang Liu,Rong Fu,Qi Tang,Yaoxia Zhang,Ruiping Cai,Limin Liu,Hui Jia,Junjia Gao,Ming-Sheng Zhou

Glia 73:2305-2321 PubMed40747821

2025

Lcn2-Induced Oligodendrocyte Ferroptosis Contributes to White Matter Damage in Chronic Cerebral Hypoperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Liu,Jiaxin Liu,Shiqin Li,Jinghan Xu,Peiqi He,Changling Li,Jinghuan Fang,Peiyan Ni,Jian Guo,Li He

Scientific reports 15:23821 PubMed40610696

2025

Differential ferroptosis regulation in red and white gastrocnemius under obesity and its Attenuation by exercise and dietary restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Fujue Ji,Haesung Lee,Hyeonseung Rheem,Jiao Liu,Jong-Hee Kim

JHEP reports : innovation in hepatology 7:101397 PubMed40524696

2025

p53 and TIGAR promote redox control to protect against metabolic dysfunction-associated steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Celine I Wittke,Eric C Cheung,Dimitris Athineos,Nicola Clements,Liam Butler,Mark Hughes,Vivienne Morrison,Dale M Watt,Karen Blyth,Karen H Vousden,Timothy J Humpton

eLife 14: PubMed40464376

2025

Targeting SLC7A11-mediated cysteine metabolism for the treatment of trastuzumab-resistant HER2-positive breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yijia Hua,Ningjun Duan,Chunxiao Sun,Fan Yang,Min Tian,Yanting Sun,Shuhan Zhao,Jue Gong,Qian Liu,Xiang Huang,Yan Liang,Ziyi Fu,Wei Li,Yongmei Yin

CNS neuroscience & therapeutics 31:e70440 PubMed40387519

2025

ALOX15-Mediated Neuron Ferroptosis Was Involved in Diabetic Peripheral Neuropathic Pain.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiye Feng,Fuye Li,Zhiqiang Lin,Jian Liu,Xi Chen,Wenxu Yan,Zhongjie Liu

Nature communications 16:2105 PubMed40025041

2025

Disruption of tryptophan metabolism by high-fat diet-triggered maternal immune activation promotes social behavioral deficits in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Penghao Sun,Mengli Wang,Xuejun Chai,Yong-Xin Liu,Luqi Li,Wei Zheng,Shulin Chen,Xiaoyan Zhu,Shanting Zhao

Scientific reports 15:1754 PubMed39799153

2025

Exploring Liraglutide's mechanism in reducing renal fibrosis: the Fsp1-CoQ10-NAD(P)H pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Chen,Ji-Xian Song,Zhi Zhang,Ji-Ren An,Yu-Jing Gou,Miao Tan,Yashuo Zhao

Nature communications 16:509 PubMed39779666

2025

S100P is a ferroptosis suppressor to facilitate hepatocellular carcinoma development by rewiring lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Min Yang,Weiwei Cui,Xiaoting Lv,Gaozhong Xiong,Caiyu Sun,Haocheng Xuan,Wei Ma,Xiuling Cui,Yeping Cheng,Lihui Han,Bo Chu

Science advances 10:eadp0443 PubMed39093974

2024

Opa1 processing is dispensable in mouse development but is protective in mitochondrial cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Sofia Ahola,Lilli A Pazurek,Fiona Mayer,Philipp Lampe,Steffen Hermans,Lore Becker,Oana V Amarie,Helmut Fuchs,Valerie Gailus-Durner,Martin Hrabe de Angelis,Dietmar Riedel,Hendrik Nolte,Thomas Langer

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Malondialdehyde antibody [11E3]

Anti-Malondialdehyde 항체 [11E3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Malondialdehyde antibody [11E3] (AB243066)

Western blot - Anti-Malondialdehyde antibody [11E3] (AB243066)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (25)

Product promise