AB223504

Anti-Mammaglobin A antibody [SP319] - C-terminal

BOND RX™ Validated
RabMAb
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(1 Publication)

Rabbit Monoclonal Mammaglobin A antibody. C-terminal. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

MGB1, UGB2, SCGB2A2, Mammaglobin-A, Mammaglobin-1, Secretoglobin family 2A member 2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mammaglobin A antibody [SP319] - C-terminal (AB223504)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mammaglobin A antibody [SP319] - C-terminal (AB223504)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Mammaglobin A with ab223504 at 1/100 dilution (1.08 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Cytoplasmic staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab223504 for 10 mins at room temperature.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mammaglobin A antibody [SP319] - C-terminal (AB223504)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Mammaglobin A with ab223504 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-Mammaglobin A antibody [SP319] - C-terminal (AB223504)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Mammaglobin A antibody [SP319] - C-terminal (AB223504)

Flow cytometric analysis of MCF7 (human breast adenocarcinoma cell line) cells stained for Mammaglobin A using ab223504 (green) at 1/100 dilution compared to negative control of rabbit IgG (blue).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP319

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "Primary Antibody Incubation: 30 minutes at 4°C.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "Antigen Retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.Primary Antibody Incubation: 10 minutes at room temperature." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

보관 버퍼

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Mammaglobin A also known as MGB1 is a breast-specific protein of approximately 93 amino acids with a molecular mass of about 15 kDa. It has a high expression in mammary tissue and limited expression in other tissues like the sweat glands. Researchers use immunohistochemistry (IHC) to detect Mammaglobin in tissue samples because of its tissue-specific expression making it a useful marker for breast epithelium.

Biological function summary

Mammaglobin A functions as a glycoprotein. Its precise biological role remains unclear but scientists propose it plays a role in secretory processes due to its presence in epithelial cells of the mammary glands. This protein itself is not known to participate in a larger complex. The significance of Mammaglobin A as a marker rather than an active enzyme or receptor suggests its potential involvement in processes specific to breast tissue.

Pathways

Mammaglobin A interacts within pathways involving sex steroid hormone signaling reflecting its expression in mammary tissue. This protein's expression is possibly regulated by hormonal pathways like the estrogen pathway which plays a significant role in breast tissue development and differentiation. It does not actively participate with related proteins but might be an indicator of pathway activities associated with specific tissues or conditions.

Mammaglobin A is prominently associated with breast cancer. Its specific expression in breast tissue makes it a valuable marker for detecting disseminated breast tumor cells. Although it does not directly interact with proteins involved in cancer progression like HER2 or ER (estrogen receptor) its expression levels serve as an important biomarker for diagnosis and monitoring treatment response. Researchers continue to explore its potential utility in understanding breast cancer biology and improving clinical outcomes.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information SCGB2A2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PloS one 20:e0315922 PubMed39813259

2025

3D fluorescence staining and confocal imaging of low amount of intestinal organoids (enteroids): Protocol accessible to all.

Applications

Unspecified application

Species

Unspecified reactive species

Ami Gloria Toulehohoun,Caroline Bouzin,Aurélie Daumerie,Luca Maccioni,Peter Stärkel

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mammaglobin A antibody [SP319] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mammaglobin A antibody [SP319] - C-terminal (AB223504)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mammaglobin A antibody [SP319] - C-terminal (AB223504)

Flow Cytometry (Intracellular) - Anti-Mammaglobin A antibody [SP319] - C-terminal (AB223504)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise