AB236550

Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Mannan Binding Lectin/MBL antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Mouse samples. Cited in 1 publication.

대체 명칭 보기

Mannose-binding protein C, MBP-C, Mannan-binding protein, RA-reactive factor P28A subunit, RARF/P28A, Mbl2

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] - BSA and Azide free (AB236550)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] - BSA and Azide free (AB236550)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Mannan Binding Lectin/MBL with ab212060 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse liver (PMID : 12538708) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212060).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unknown

Immunoprecipitation - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] - BSA and Azide free (AB236550)

Mannan Binding Lectin/MBL was immunoprecipitated from 0.35 mg of mouse serum with ab212060 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab212060 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Mouse serum 10 μg (Input).

Lane 2 : ab212060 IP in mouse serum.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab212060 in mouse serum.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab212060).

All lanes:

Immunoprecipitation - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] (<a href='/ko/products/primary-antibodies/mannan-binding-lectin-mbl-antibody-epr18381-83-ab212060'>ab212060</a>)

Predicted band size: 26 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18381-83

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab236550 is the carrier-free version of ab212060.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Mannan Binding Lectin often referred to as MBL is an important player in the innate immune system. It is known alternatively as mannose-binding lectin or mannan-binding protein. The mass of MBL is approximately 32 to 34 kilodaltons and it is primarily produced in the liver. MBL circulates in the bloodstream where it can recognize patterns on the surface of pathogens such as bacteria and viruses through its lectin-binding domain. This recognition leads to the activation of other components that play roles in immune response.

Biological function summary

Mannan Binding Lectin serves a significant role in immune defense by binding to carbohydrate structures on pathogens. It is part of a complex known as the lectin pathway of the complement system one of three pathways that activate the complement system to enhance pathogen removal. MBL can activate through a series of proteases leading to opsonization cell lysis and inflammation which are vital responses to infection.

Pathways

Mannan recognition by MBL initiates the lectin pathway an essential component of the complement system. This pathway converges with the classical and alternative pathways to enhance the immune response. MBL works closely with MBL-associated serine proteases (MASPs) particularly MASP-1 and MASP-2 which cleave complement proteins resulting in pathogen opsonization and membrane attack complex formation. These actions are tightly regulated and ensure effective defense against invaders.

Deficiencies or polymorphisms in MBL can lead to increased susceptibility to infections and developmental complications of the immune system. Individuals with low MBL levels may experience recurrent respiratory infections or other bacterial infections due to inadequate complement activation. Moreover variations in the MBL protein have connections with autoimmune diseases such as systemic lupus erythematosus where MBL levels might influence disease severity. In these conditions altered interactions with other immune proteins like C4 and C3 may contribute to pathological responses.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity).

See full target information Mbl2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 14:7136 PubMed37932296

2023

FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Zongchao Sun,Likun Yang,Abdukahar Kiram,Jing Yang,Zhuangzhuang Yang,Liwei Xiao,Yujing Yin,Jing Liu,Yan Mao,Danxia Zhou,Hao Yu,Zheng Zhou,Dengqiu Xu,Yuhuan Jia,Chenyun Ding,Qiqi Guo,Hongwei Wang,Yan Li,Li Wang,Tingting Fu,Shijun Hu,Zhenji Gan

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] - BSA and Azide free (AB236550)

Immunoprecipitation - Anti-Mannan Binding Lectin/MBL antibody [EPR18381-83] - BSA and Azide free (AB236550)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise