Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Immunohistochemical analysis of paraffin-embedded Human cerebrum labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on Human cerebrum. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Immunohistochemical analysis of paraffin-embedded Human pancreas labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on islet in the human pancreas. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Immunohistochemical analysis of paraffin-embedded Human colon labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on ganglion in the human colon. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Composite multiplex immunofluorescence staining of Iba1, GFAP and MAP2 staining in a section of formalin-fixed paraffin-embedded human cerebral cortex*.

Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with EDTA (Ph9.0) using retrieval settings of 100°C for 40 minutes. The section was then incubated at room temperature for 1 hour with ab323239 at 5µg/ml dilution (shown in green), ab300645 at 5µg/ml (shown in magenta), and ab178846 at 5µg/ml (shown in yellow). Then incubated for 1 hour with ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed 1/1000, ab150084 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed 1/1000, and ab150173 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) preadsorbed 1/1000. Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium ^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Immunohistochemical analysis of paraffin-embedded Rat colon labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on ganglion in the rat colon. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

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Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat cerebellum tissue labeling MAP2 with ab300645 at 1/100 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (green). Positive staining on rat cerebellum is observed. The nuclear counter stain is DAPI (blue). Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. No antigen retrieval was carried out.

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized rat primary neural/glia cells labeling MAP2 with ab300645 at 1/100 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 488) pre-adsorbed at 1/1000 dilution (green). Counterstain was ab183830 Anti-MAP2 rabbit monoclonal antibody at 1/1000 dilution along with ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) as the counterstain secondary used at 1/1000 dilution. Confocal image showing cytoplasmic staining in rat primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The nuclear counter stain is DAPI (blue).  Negative control 1 : ab300645 at 1/100 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution. Negative control 2 : ab183830 at 1/1000 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 488) pre-adsorbed at 1/1000 dilution.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Immunohistochemical analysis of paraffin-embedded Mouse colon labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on ganglion in the mouse colon. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling MAP2 with ab300645 at 1/100 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 488) pre-adsorbed at 1/1000 dilution (green). Counterstain was ab183830 Anti-MAP2 rabbit monoclonal antibody at 1/1000 dilution along with ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) as the counterstain secondary used at 1/1000 dilution. Confocal image showing cytoplasmic staining in mouse primary neuron. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. The nuclear counter stain is DAPI (blue). Negative control 1 : ab300645 at 1/100 dilution followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at 1/1000 dilution. Negative control 2 : ab183830 at 1/1000 dilution followed by ab98736 Goat F(ab')2 Anti-Mouse IgG - Fc (DyLight® 488) pre-adsorbed at 1/1000 dilution.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse cerebellum tissue labeling MAP2 with ab300645 at 1/100 dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (green). Positive staining on mouse cerebellum is observed. The nuclear counter stain is DAPI (blue). Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. No antigen retrieval was carried out.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on Rat cerebrum. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR19691] - Neuronal Marker - Mouse IgG2a (Chimeric) (AB300645)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum labeling MAP2 with ab300645 at 1/10000 dilution (0.086 µg/ml) followed by a ready to use Leica DS9800 (BOND™ Polymer Refine Detection) kit. Positive staining on Mouse cerebrum. The section was incubated with ab300645 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins