Anti-MAP2 antibody [EPR22036-127] - Neuronal Marker

• IP

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Immunoprecipitation - Anti-MAP2 antibody [EPR22036-127] - Neuronal Marker (AB221693)

MAP2 was immunoprecipitated from 0.35 mg SK-N-BE(2) (Human neuroblastoma neuroblast) whole cell lysate 10 ug with ab221693 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab221693 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : SK-N-BE(2) (Human neuroblastoma neuroblast) whole cell lysate 10 ug

Lane 2 : ab221693 IP in SK-N-BE(2) whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab221693 in SK-N-BE(2) whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds

Fresh made lysate is required to minimize protein degradation.

All lanes:

Immunoprecipitation - Anti-MAP2 antibody [EPR22036-127] - Neuronal Marker (ab221693)

Predicted band size: 199 kDa

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• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-MAP2 antibody [EPR22036-127] - Neuronal Marker (AB221693)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse brain cells cells labelling MAP2 with ab221693 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] - Neuronal Marker (AB221693)

Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse liver tissue labeling MAP2 using ab221693 at 1/1000 dilution, followed by an AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Negative control : No staining on mouse liver (PMID : 7001466, PMID : 2423532). is observed. The nuclear counterstain is DAPI (Blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] - Neuronal Marker (AB221693)

Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse cerebrum tissue labeling MAP2 (Green) using ab221693 at 1/1000 dilution, followed by an AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Cytoplasmic staining on mouse cerebrum (PMID : 22479190) is observed. The nuclear counterstain is DAPI (Blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22036-127] - Neuronal Marker (AB221693)

Immunohistochemical analysis of frozen 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse kidney tissue labeling MAP2 using ab221693 at 1/1000 dilution, followed by an AlexaFluor^®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution. Negative control : No staining on mouse kidney (PMID : 7001466, PMID : 2423532) is observed. The nuclear counterstain is DAPI (Blue).

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22036-127] - Neuronal Marker (AB221693)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling MAP2 with ab221693 at 1/1000 dilution, followed by AlexaFluor^®488 Goat anti-Rabbit (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cells is observed. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) was used as a counterstain at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Negative control : Hepa1-6 cells (PMID : 7001466)