Anti-MAP2 antibody [EPR22641-106] - Neuronal Marker

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-MAP2 antibody [EPR22641-106] - Neuronal Marker (AB254263)

Fluorescence multiplex immunohistochemical analysis of the human cerebrum (Formalin/PFA-fixed paraffin-embedded sections).

Panel A : merged staining of anti-GFAP (magenta; Opal™690), anti-MAP2 (green; Opal™520) and anti-GPCR GPR17 (red; Opal™570) on human cerebrum.
Panel B : anti-MAP2 staining neurons.
Panel C : anti-GPCR GPR17 staining oligodendrocytes.
Panel D : anti-GFAP staining astrocytes.

The section was incubated in three rounds of staining : in the order of ab254263, ab314307, and ab68428 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-106] - Neuronal Marker (AB254263)

Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling MAP2 with ab254263 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human glioma (PMID : 28766965, 15642108) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-106] - Neuronal Marker (AB254263)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MAP2 with ab254263 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in neuron of human colon (PMID : 28766965, 15642108) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-106] - Neuronal Marker (AB254263)

Fluorescence multiplex immunohistochemical analysis of the Human cerebellum (Formalin/PFA-fixed paraffin-embedded sections).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Opal Polymer HRP Ms + Rb was used as a secondary antibody.DAPI (blue) was used as a nuclear counter stain.

Panel A : Merged staining of anti-GFAP (gray; Opal™690), anti-P2Y12 (green; Opal™520) and anti-MAP2 (red; Opal™570) on human cerebellum. Panel B : Anti-MAP2 stained cell body and dendrites of neurons. Panel C : Anti-P2Y12 stained on microglial cells. Panel D : Anti-GFAP stained on astrocytes.

The section was incubated in three rounds of staining : in the order of ab68428, ab254347, and ab254263 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-106] - Neuronal Marker (AB254263)

Fluorescence multiplex immunohistochemical analysis of the Human cerebrum (Formalin/PFA-fixed paraffin-embedded sections).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Opal Polymer HRP Ms + Rb was used as a secondary antibody.DAPI (blue) was used as a nuclear counter stain.

Panel A : Merged staining of anti-GFAP (gray; Opal™690), anti-P2Y12 (green; Opal™520) and anti-MAP2 (red; Opal™570) on human cerebrum. Panel B : Anti-MAP2 stained cell body and dendrites of neurons. Panel C : Anti-P2Y12 stained on microglial cells. Panel D : Anti-GFAP stained on astrocytes.

The section was incubated in three rounds of staining : in the order of ab68428, ab254347, and ab254263 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-106] - Neuronal Marker (AB254263)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAP2 with ab254263 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human cerebrum (PMID : 28766965, 15642108) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-MAP2 antibody [EPR22641-106] - Neuronal Marker (AB254263)

Fluorescence multiplex immunohistochemical analysis of human cerebellum tissue (formalin/PFA-fixed paraffin-embedded section). Panel A : merged staining of anti-GFAP (ab68428, gray; Opal™690), anti-Myelin PLP (ab254363, green; Opal™520) and anti-MAP2 (ab254263, red; Opal™570) on human cerebellum tissue. Panel B : anti-MAP2 stained cell body and dendrites of neurons. Panel C : anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D : anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab68428 (1/50 dilution), ab254363 (1/2000 dilution), and ab254263 (1/4000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-MAP2 antibody [EPR22641-106] - Neuronal Marker (AB254263)

Fluorescence multiplex immunohistochemical analysis of human cerebrum tissue (formalin/PFA-fixed paraffin-embedded section). Panel A : merged staining of anti-GFAP (ab68428, gray; Opal™690), anti-Myelin PLP (ab254363, green; Opal™520) and anti-MAP2 (ab254263, red; Opal™570) on human cerebrum tissue. Panel B : anti-MAP2 stained cell body and dendrites of neurons. Panel C : anti-Myelin PLP stained on myelin sheaths of oligodendrocytes. Panel D : anti-GFAP stained on astrocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND^®RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining : in the order of ab68428 (1/50 dilution), ab254363 (1/2000 dilution), and ab254263 (1/4000 dilution) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.

Image acquisition was performed with Leica SP8 confocal microscope.