Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker (AB254264)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in neuron of human colon (PMID : 28766965,15642108) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker (AB254264)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in human cerebrum (PMID : 28766965,15642108) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker (AB254264)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling MAP2 with ab254264 at 1/2000 (0.299 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron.Confocal scanning Z step was set as 0.3 µm followed by image processing with maximum Z projection. ab11267 Anti-MAP2 mouse monoclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2ug/ml dilution.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker (AB254264)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebral cortex tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on rat cerebral cortex (PMID : 22275884) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker (AB254264)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in rat cerebrum (PMID : 28766965,15642108) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker (AB254264)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebral cortex tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on mouse cerebral cortex (PMID : 22275884) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker (AB254264)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling MAP2 with ab254264 at 1/4000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in mouse cerebrum (PMID : 28766965,15642108) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker (AB254264)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on mouse cerebellum (PMID : 22275884) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker (AB254264)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue staining SLC7A5/LAT1 with ab324354 at a 1/2000 dilution, ab236870 anti-NeuN/RBFOX3 used at 1/10000 dilution and ab254264 anti-MAP2 used at a 1/4000 dilution.

Panel A : anti-SLC7A5/LAT1 (green; Opal™520), anti-NeuN/RBFOX3 (magenta; Opal™690) and anti-MAP2 (gray; Opal™570) on mouse cerebrum.

Panel B : anti-SLC7A5/LAT1 staining blood vessels in mouse cerebrum.

Panel C : anti-NeuN/RBFOX3 staining nucleus of neurons in mouse cerebrum.

Panel D : anti-MAP2 staining cell body and dendrites of neurons in mouse cerebrum.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324354, ab236870 and ab254264 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker (AB254264)

Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling MAP2 with ab254264 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive staining on rat cerebellum (PMID : 22275884) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-MAP2 antibody [EPR22641-16] - Neuronal Marker (AB254264)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse hippocampus tissue staining SLC7A5/LAT1 with ab324354 at a 1/2000 dilution, ab236870 anti-NeuN/RBFOX3 used at 1/10000 dilution and ab254264 anti-MAP2 used at a 1/4000 dilution.

Panel A : anti-SLC7A5/LAT1 (green; Opal™520), anti-NeuN/RBFOX3 (magenta; Opal™690) and anti-MAP2 (gray; Opal™570) on mouse hippocampus.

Panel B : anti-SLC7A5/LAT1 staining blood vessels in mouse hippocampus.

Panel C : anti-NeuN/RBFOX3 staining nucleus of neurons in mouse hippocampus.

Panel D : anti-MAP2 staining cell body and dendrites of neurons in mouse hippocampus.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324354, ab236870 and ab254264 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.