AB96378

Anti-MAP2 (phospho S136) antibody [EPR2361]

Name: Anti-MAP2 (phospho S136) antibody [EPR2361]
Brand: Abcam Limited
SKU: AB96378
Rating: 5 (2 reviews)

20ul selling size
RabMAb
Recombinant
Lab Essentials
각 태그의 뜻

(2 리뷰들)

(10 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal MAP2 phospho S136 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 10 publications.

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 (phospho S136) antibody [EPR2361] (AB96378)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 (phospho S136) antibody [EPR2361] (AB96378)

ab96378, at a 1/100 dilution, staining MAP2 in paraffin embedded Human brain tissue by Immunohistochemical analysis.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 (phospho S136) antibody [EPR2361] (AB96378)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 (phospho S136) antibody [EPR2361] (AB96378)

Immunocytochemistry/ Immunofluorescence analysis of mouse primary neuron + Alkaline Phosphatase cells labeling MAP2 with purified ab96378 at 1/500 (3 μg/mL). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

Unknown

Western blot - Anti-MAP2 (phospho S136) antibody [EPR2361] (AB96378)

All lanes:

Western blot - Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378) at 1/1000 dilution

Lane 1:

Untreated fetal brain lysates at 10 µg

Lane 2:

Fetal brain lysates treated with Alkaline Phosphatase at 10 µg

Lane 3:

Mouse brain lysates at 10 µg

Secondary

All lanes:

HRP/AP polymerized antibody

Predicted band size: 199 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2361

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Stable for 12 months at -20°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.

See full target information MAP2 pS136

대체 명칭 보기

Microtubule-associated protein 2, MAP-2, MAP2

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제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록and refine your search

Scientific reports 15:29738 PubMed40804138

2025

Functional discrimination of CSF from Alzheimer's patients in a brain on chip platform.

Applications

Unspecified application

Species

Unspecified reactive species

Louise Miny,Jessica Rontard,Ahmad Allouche,Nicolas Violle,Louise Dubuisson,Aurélie Batut,Alexandre Ponomarenko,Rania Talbi,Hélène Gautier,Benoît G C Maisonneuve,Serge Roux,Florian Larramendy,Thibault Honegger,Isabelle Quadrio

Journal of orthopaedic surgery and research 20:310 PubMed40128722

2025

Electroacupuncture attenuates intervertebral disc degeneration by upregulating aquaporins via the cAMP/PKA pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Jia-Bao Huang,Jing Zou,Guo-Fu Huang

British journal of pharmacology 181:2012-2032 PubMed38112022

2023

A novel role for microtubule affinity-regulating kinases in neuropathic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Qin Shi,Zhi-Hong Sun,Zhe-Zhe Wang,Chun-Yu Su,Wei Zhang,Lin-Yao Yu,Yang Xu,Yong-Lin Gao,Hong-Bo Wang,Jing-Wei Tian,Chun-Mei Li

Cell death discovery 9:297 PubMed37582760

2023

NeuroD4 converts glioblastoma cells into neuron-like cells through the SLC7A11-GSH-GPX4 antioxidant axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Wang,Peiqi Zhao,Ying Zhang,Zhen Chen,Han Bao,Wenqi Qian,Jian Wu,Zhenqiu Xing,Xiaowei Hu,Kunlin Jin,Qichuan Zhuge,Jianjing Yang

International journal of molecular sciences 24: PubMed37445748

2023

Proof-of-Concept Human Organ-on-Chip Study: First Step of Platform to Assess Neuro-Immunological Communication Involved in Inflammatory Bowel Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Tristan Gabriel-Segard,Jessica Rontard,Louise Miny,Louise Dubuisson,Aurélie Batut,Delphine Debis,Mélanie Gleyzes,Fabien François,Florian Larramendy,Alessandra Soriano,Thibault Honegger,Stéphane Paul

Theranostics 12:3847-3861 PubMed35664063

2022

PTBP1 knockdown promotes neural differentiation of glioblastoma cells through UNC5B receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Kankai Wang,Sishi Pan,Peiqi Zhao,Li Liu,Zhen Chen,Han Bao,Hao Wang,Ying Zhang,Qichuan Zhuge,Jianjing Yang

PLoS pathogens 15:e1008249 PubMed31887215

2019

Cross-talk between microglia and neurons regulates HIV latency.

Applications

Unspecified application

Species

Unspecified reactive species

David Alvarez-Carbonell,Fengchun Ye,Nirmala Ramanath,Yoelvis Garcia-Mesa,Pamela E Knapp,Kurt F Hauser,Jonathan Karn

EBioMedicine 42:174-187 PubMed30926423

2019

Toxic amyloid-β oligomers induced self-replication in astrocytes triggering neuronal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wang,Ting-Ting Hou,Long-Fei Jia,Qiao-Qi Wu,Mei-Na Quan,Jian-Ping Jia

Cerebral cortex (New York, N.Y. : 1991) 29:3738-3751 PubMed30307479

2018

Isozyme-Specific Role of SAD-A in Neuronal Migration During Development of Cerebral Cortex.

Applications

Unspecified application

Species

Unspecified reactive species

Keiko Nakanishi,Hiroyuki Niida,Hidenori Tabata,Tsuyoshi Ito,Yuki Hori,Madoka Hattori,Yoshikazu Johmura,Chisato Yamada,Takashi Ueda,Kosei Takeuchi,Kenichiro Yamada,Koh-Ichi Nagata,Nobuaki Wakamatsu,Masashi Kishi,Y Albert Pan,Shinya Ugawa,Shoichi Shimada,Joshua R Sanes,Yujiro Higashi,Makoto Nakanishi

Scientific reports 4:6671 PubMed25327364

2014

Effects of let-7b and TLX on the proliferation and differentiation of retinal progenitor cells in vitro.

Applications

ICC

Species

Mouse

Ni Ni,Dandan Zhang,Qing Xie,Junzhao Chen,Zi Wang,Yuan Deng,Xuyang Wen,Mengyu Zhu,Jing Ji,Xianqun Fan,Min Luo,Ping Gu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAP2 (phospho S136) antibody [EPR2361]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 (phospho S136) antibody [EPR2361] (AB96378)

Immunocytochemistry/ Immunofluorescence - Anti-MAP2 (phospho S136) antibody [EPR2361] (AB96378)

Western blot - Anti-MAP2 (phospho S136) antibody [EPR2361] (AB96378)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (10)

Product promise