AB33910

Anti-MAP4K1/HPK1 antibody [EP630Y]

RabMAb
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(8 제품이 사용된 논문 )

Rabbit Monoclonal MAP4K1/HPK1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 8 publications.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K1/HPK1 antibody [EP630Y] (AB33910)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K1/HPK1 antibody [EP630Y] (AB33910)

ab33910 staining MAP4K1/HPK1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

Negative control 1 : PBS in place of primary antibody.

Lab

Western blot - Anti-MAP4K1/HPK1 antibody [EP630Y] (AB33910)

Blocking buffer : 5% NFDM/TBST

Diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-MAP4K1/HPK1 antibody [EP630Y] (ab33910) at 1/5000 dilution

Lane 1:

Daudi (human Burkitt's lymphoma) whole cell lysate at 20 µg

Lane 2:

Raji (human Burkitt's lymphoma) whole cell lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/20000 dilution

Predicted band size: 91 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP630Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab33910 recognises hematopoietic progenitor kinase 1 (HPK1).

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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제품 세부 정보

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.09% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Serine/threonine-protein kinase, which plays a role in the response to environmental stress (PubMed : 24362026). Appears to act upstream of the JUN N-terminal pathway (PubMed : 8824585). Activator of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. MAP4Ks act in parallel to and are partially redundant with STK3/MST2 and STK4/MST2 in the phosphorylation and activation of LATS1/2, and establish MAP4Ks as components of the expanded Hippo pathway (PubMed : 26437443). May play a role in hematopoietic lineage decisions and growth regulation (PubMed : 24362026, PubMed : 8824585). Together with CLNK, it enhances CD3-triggered activation of T-cells and subsequent IL2 production (By similarity).

See full target information MAP4K1

대체 명칭 보기

HPK1, MAP4K1, Mitogen-activated protein kinase kinase kinase kinase 1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEK kinase kinase 1, MEKKK 1

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제품이 사용된 논문 (8)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of medicinal chemistry 67:17429-17453 PubMed39331123

2024

Discovery of BAY-405: An Azaindole-Based MAP4K1 Inhibitor for the Enhancement of T-Cell Immunity against Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey Mowat,Rafael Carretero,Gabriele Leder,Nuria Aiguabella Font,Roland Neuhaus,Sandra Berndt,Judith Günther,Anders Friberg,Martina Schäfer,Hans Briem,Marian Raschke,Hideki Miyatake Ondozabal,Bernd Buchmann,Ulf Boemer,Bertolt Kreft,Ingo V Hartung,Rienk Offringa

Nature biomedical engineering 7:1063-1080 PubMed37069267

2023

A lentiviral vector for the production of T cells with an inducible transgene and a constitutively expressed tumour-targeting receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick Reichenbach,Greta Maria Paola Giordano Attianese,Khaoula Ouchen,Elisabetta Cribioli,Melanie Triboulet,Sarah Ash,Margaux Saillard,Romain Vuillefroy de Silly,George Coukos,Melita Irving

Frontiers in oncology 9:523 PubMed31293967

2019

Comprehensive Gene Expression Analysis in NMIBC Using RNA-seq Reveals New Therapy Strategies.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Chen,Fuquan Jiang,Chunshu Jia,Ming Liu,Yonghao Nan,Licheng Qu,Qingkuo Kong,Fangfang Hou,Wenshan Luo,Wanli Na,Xuefei Jin,Jiufeng Tan

Nature communications 10:1547 PubMed30948712

2019

Identification of the kinase STK25 as an upstream activator of LATS signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Sanghee Lim,Nicole Hermance,Tenny Mudianto,Hatim M Mustaly,Ian Paolo Morelos Mauricio,Marc A Vittoria,Ryan J Quinton,Brian W Howell,Hauke Cornils,Amity L Manning,Neil J Ganem

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:7037-7048 PubMed30870006

2019

Long noncoding RNA CDKN2B-AS1 interacts with transcription factor BCL11A to regulate progression of cerebral infarction through mediating MAP4K1 transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Jie Lei,Hui-Qing Li,Zhi-Huai Mo,Ke-Jia Liu,Ling-Juan Zhu,Chun-Yi Li,Wen-Li Chen,Lei Zhang

Cell death and differentiation 26:1346-1364 PubMed30341421

2018

Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Chen,Feng Yang,Erhu Fang,Wenjing Xiao,Hong Mei,Huanhuan Li,Dan Li,Huajie Song,Jianqun Wang,Mei Hong,Xiaojing Wang,Kai Huang,Liduan Zheng,Qiangsong Tong

International journal of molecular medicine 38:803-11 PubMed27430936

2016

Ubiquitin-specific protease 4 inhibits breast cancer cell growth through the upregulation of PDCD4.

Applications

Unspecified application

Species

Human

Yang Li,Daqing Jiang,Qi Zhang,Xiaoli Liu,Zhengang Cai

Cancer research 68:4239-47 PubMed18519683

2008

Pediatric malignant germ cell tumors show characteristic transcriptome profiles.

Applications

IHC-P

Species

Human

Roger D Palmer,Nuno L Barbosa-Morais,Emma L Gooding,Balaji Muralidhar,Claire M Thornton,Mark R Pett,Ian Roberts,Dominik T Schneider,Natalie Thorne,Simon Tavaré,James C Nicholson,Nicholas Coleman

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAP4K1/HPK1 antibody [EP630Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP4K1/HPK1 antibody [EP630Y] (AB33910)

Western blot - Anti-MAP4K1/HPK1 antibody [EP630Y] (AB33910)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (8)

Product promise