AB74032

Anti-MAPK6/ERK3 (phospho S189) antibody

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(9 제품이 사용된 논문 )

Rabbit Polyclonal MAPK6/ERK3 phospho S189 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human MAPK6 pS189.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 (phospho S189) antibody (AB74032)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 (phospho S189) antibody (AB74032)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ab74032, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising phosphopeptide.

Unknown

Western blot - Anti-MAPK6/ERK3 (phospho S189) antibody (AB74032)

All lanes:

Western blot - Anti-MAPK6/ERK3 (phospho S189) antibody (ab74032) at 1/500 dilution

Lane 1:

Extracts from mouse brain at 5 µg

Lane 2:

Extracts from mouse brain at 5 µg with immunising phosphopeptide

Predicted band size: 83 kDa

Observed band size: 90 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MAPK6 pS189. The exact immunogen used to generate this antibody is proprietary information.

Q16659

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab74032 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events : upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity).

See full target information MAPK6 pS189

대체 명칭 보기

ERK3, PRKM6, MAPK6, Mitogen-activated protein kinase 6, MAP kinase 6, MAPK 6, Extracellular signal-regulated kinase 3, MAP kinase isoform p97, ERK-3, p97-MAPK

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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular metabolism 96:102134 PubMed40189102

2025

C3aR1 on β cells enhances β cell function and survival to maintain glucose homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Renan Pereira de Lima,Ang Li,Ankit Gilani,Alfonso Rubio-Navarro,Charles D Warren,Isabella Y Kong,Jacob B Geri,James C Lo

Cell death & disease 13:500 PubMed35614037

2022

Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Brandolini,Michele d'Angelo,Rubina Novelli,Vanessa Castelli,Cristina Giorgio,Anna Sirico,Pasquale Cocchiaro,Francesco D'Egidio,Elisabetta Benedetti,Claudia Cristiano,Antonella Bugatti,Anna Ruocco,Pier Giorgio Amendola,Carmine Talarico,Candida Manelfi,Daniela Iaconis,Andrea Beccari,Andreza U Quadros,Thiago M Cunha,Arnaldo Caruso,Roberto Russo,Annamaria Cimini,Andrea Aramini,Marcello Allegretti

The European journal of neuroscience 55:1471-1482 PubMed35243702

2022

Inhibition of ERK1/2 regulates cognitive function by decreasing expression levels of PSD-95 in the hippocampus of CIH rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Huang,Pei Wang,Haijun Liu,Mingjian Li,Yujiao Yue,Ping Xu

American journal of physiology. Cell physiology : PubMed33502949

2021

Suppression of lncRNA HOTAIR alleviates RCC angiogenesis through regulating miR-126/EGFL7 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Ping Guo,Zhi-Feng Wang,Na Li,Qian-Qian Lei,Qiong Cheng,Li-Gang Shi,Sheng-Li Zhou,Xin-Hua Wang,Yang Sun,Ling-Fei Kong

Cell cycle (Georgetown, Tex.) 19:1022-1035 PubMed32208888

2020

Exosomes-carried microRNA-26b-5p regulates microglia M1 polarization after cerebral ischemia/reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Guangying Li,Longhai Xiao,Hao Qin,Qiang Zhuang,Weiwei Zhang,Long Liu,Chao Di,Yabo Zhang

Bioscience reports 40: PubMed32202300

2020

MiR-148a inhibits oral squamous cell carcinoma progression through ERK/MAPK pathway via targeting IGF-IR.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Jia,Yipeng Ren,Fengze Wang,Rui Zhao,Bo Qiao,Lejun Xing,Long Ou,Bin Guo

Cell cycle (Georgetown, Tex.) 18:3404-3419 PubMed31650885

2019

Runt-related transcription factor 1 contributes to lung cancer development by binding to tartrate-resistant acid phosphatase 5.

Applications

Unspecified application

Species

Unspecified reactive species

Changjun He,Xue Bai,Yingbin Li,Haobo Sun,Xianglong Kong,Bicheng Fu,Lantao Chen,Kaibin Zhu,Pengju Li,Shidong Xu

Archives of oral biology 102:164-170 PubMed31055249

2019

Over-expression of Bcl2-associated athanogene 2 in oral cancer promotes cellular proliferation and is associated with poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Song Liu,Bing Wei

Journal of hematology & oncology 10:59 PubMed28241849

2017

Metformin potentiates the effect of arsenic trioxide suppressing intrahepatic cholangiocarcinoma: roles of p38 MAPK, ERK3, and mTORC1.

Applications

Unspecified application

Species

Unspecified reactive species

Sunbin Ling,Haiyang Xie,Fan Yang,Qiaonan Shan,Haojiang Dai,Jianyong Zhuo,Xuyong Wei,Penghong Song,Lin Zhou,Xiao Xu,Shusen Zheng

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MAPK6/ERK3 (phospho S189) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAPK6/ERK3 (phospho S189) antibody (AB74032)

Western blot - Anti-MAPK6/ERK3 (phospho S189) antibody (AB74032)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (9)

Product promise