AB136872

Anti-MARK2 antibody - N-terminal

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(4 제품이 사용된 논문 )

Rabbit Polyclonal MARK2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human MARK2.

대체 명칭 보기

EMK1, MARK2, Serine/threonine-protein kinase MARK2, ELKL motif kinase 1, MAP/microtubule affinity-regulating kinase 2, PAR1 homolog, PAR1 homolog b, EMK-1, Par-1b, Par1b

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-MARK2 antibody - N-terminal (AB136872)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MARK2 antibody - N-terminal (AB136872)

Immunofluorescence of A549 cells labelling ab136872 at 1/100 dilution. Image on the right is treated with synthesised peptide

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARK2 antibody - N-terminal (AB136872)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARK2 antibody - N-terminal (AB136872)

Immunohistochemical analysis of paraffin embedded Human kidney tubules labelling ab136872 at 10µg/ml.

Unknown

Western blot - Anti-MARK2 antibody - N-terminal (AB136872)

All lanes:

Western blot - Anti-MARK2 antibody - N-terminal (ab136872) at 1/500 dilution

Lane 1:

COS7 cell extract

Lane 2:

COS7 cell extract with synthesised peptide

Predicted band size: 88 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, African green monkey

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MARK2. The exact immunogen used to generate this antibody is proprietary information.

Q7KZI7

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Serine/threonine-protein kinase (PubMed : 23666762). Involved in cell polarity and microtubule dynamics regulation. Phosphorylates CRTC2/TORC2, DCX, HDAC7, KIF13B, MAP2, MAP4 and RAB11FIP2. Phosphorylates the microtubule-associated protein MAPT/TAU (PubMed : 23666762). Plays a key role in cell polarity by phosphorylating the microtubule-associated proteins MAP2, MAP4 and MAPT/TAU at KXGS motifs, causing detachment from microtubules, and their disassembly. Regulates epithelial cell polarity by phosphorylating RAB11FIP2. Involved in the regulation of neuronal migration through its dual activities in regulating cellular polarity and microtubule dynamics, possibly by phosphorylating and regulating DCX. Regulates axogenesis by phosphorylating KIF13B, promoting interaction between KIF13B and 14-3-3 and inhibiting microtubule-dependent accumulation of KIF13B. Also required for neurite outgrowth and establishment of neuronal polarity. Regulates localization and activity of some histone deacetylases by mediating phosphorylation of HDAC7, promoting subsequent interaction between HDAC7 and 14-3-3 and export from the nucleus. Also acts as a positive regulator of the Wnt signaling pathway, probably by mediating phosphorylation of dishevelled proteins (DVL1, DVL2 and/or DVL3). Modulates the developmental decision to build a columnar versus a hepatic epithelial cell apparently by promoting a switch from a direct to a transcytotic mode of apical protein delivery. Essential for the asymmetric development of membrane domains of polarized epithelial cells.

See full target information MARK2

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell communication and signaling : CCS 23:263 PubMed40462136

2025

Enhancing intestinal epithelial microtubule stability could alleviate IBD symptoms.

Applications

Unspecified application

Species

Unspecified reactive species

Fangying Yang,Zhengxiao He,Xiumei Pan,Shuping Xie,Xinhua Liang,Lanlan Geng,Wanfu Xu,Sitang Gong

eLife 14: PubMed40333320

2025

MARK2 regulates Golgi apparatus reorientation by phosphorylation of CAMSAP2 in directional cell migratio.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Xu,Rui Zhang,Zhengrong Zhou,Honglin Xu,Yuejia Li,Mengge Yang,Ruifan Lin,Yingchun Wang,Xiahe Huang,Qi Xie,Wenxiang Meng

Current biology : CB 32:2704-2718.e6 PubMed35594862

2022

MARK2 regulates directed cell migration through modulation of myosin II contractility and focal adhesion organization.

Applications

Unspecified application

Species

Unspecified reactive species

Ana M Pasapera,Sarah M Heissler,Masumi Eto,Yukako Nishimura,Robert S Fischer,Hawa R Thiam,Clare M Waterman

Molecular therapy. Nucleic acids 8:277-290 PubMed28918029

2017

Activation of AMPK Attenuated Cardiac Fibrosis by Inhibiting CDK2 via p21/p27 and miR-29 Family Pathways in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Hanping Qi,Yan Liu,Shuzhi Li,Yunping Chen,Lei Li,Yonggang Cao,Mingyao E,Pilong Shi,Chao Song,Baiyan Li,Hongli Sun

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MARK2 antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-MARK2 antibody - N-terminal (AB136872)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARK2 antibody - N-terminal (AB136872)

Western blot - Anti-MARK2 antibody - N-terminal (AB136872)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise