AB235198

Anti-MASH1/Achaete-scute homolog 1 antibody

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Goat Polyclonal MASH1/Achaete-scute homolog 1 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

대체 명칭 보기

ASH1, BHLHA46, HASH1, ASCL1, Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody (AB235198)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody (AB235198)

Human thyroid papillary carcinoma tissue stained for MASH1/Achaete-scute homolog 1 with ab235198 at a 1/1000 dilution in immunohistochemical analysis. Counterstained with hematoxylin. Antigen retrieval HIER pH 6. No fixative.

Supplier Data

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (AB235198)

All lanes:

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab235198) at 1/1000 dilution

Lane 1:

Mouse pancreas lysate at 35 µg

Lane 2:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 35 µg

Secondary

All lanes:

Anti-Goat IgG (H&L) (Rabbit) peroxidase conjugated at 1/5000 dilution

Predicted band size: 25 kDa

false

Supplier Data

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (AB235198)

All lanes:

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab235198) at 1/1000 dilution

All lanes:

MASH1/Achaete-scute homolog 1 human recombinant protein C-MYC/DDK-tagged, 50 ng

Secondary

All lanes:

Donkey Fab2-anti-Goat [H&L] mx8 HRP at 1/5000 dilution

Predicted band size: 25 kDa

false

Exposure time: 15s

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The MASH1 protein also known as Achaete-Scute homolog 1 (ASCL1) plays an important role in neurogenesis. It acts as a transcription factor and regulates neuronal differentiation. MASH1 has a molecular weight of approximately 34 kDa. It expresses predominantly in the nervous system including the developing neural tube and peripheral ganglia and it can also be found in certain regions of the brain during embryonic development. ASCL1 is part of the basic helix-loop-helix (bHLH) family of transcription factors which are key regulators of neuronal identity and function.

Biological function summary

This protein regulates the expression of genes important for the development of neuronal precursors. MASH1 does not act alone; it forms heterodimers with other bHLH proteins which enhance its ability to bind DNA and influence gene transcription. It helps convert multipotent neural progenitors into committed neuronal precursors which later differentiate into specific neuronal cell types. MASH1's role is particularly important during early stages of neuronal differentiation ensuring proper formation and maturation of the nervous system.

Pathways

MASH1 is central to the Notch signaling pathway and other pathways involved in neural differentiation. Within the Notch pathway MASH1 interacts with proteins like HES1 to balance the differentiation of neural progenitor cells into neurons and glial cells. This protein also connects with other pathway components such as NEUROG2 a downstream target to promote neuronal fate specification. These interactions illustrate MASH1’s integration into complex signaling networks that govern cell fate decisions during neurogenesis.

MASH1 has significant associations with neurodevelopmental disorders and certain cancers. Aberrant ASCL1 activity links to conditions like Hirschsprung's disease where neural crest development is impaired and some types of lung cancer particularly pulmonary neuroendocrine tumors. It is known to interact with the RET protein in the context of Hirschsprung's disease and with proteins like MYC in cancer pathways highlighting its multifaceted involvement in pathology. Understanding these associations helps elucidate the mechanisms of these diseases and supports therapeutic target development.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription factor that plays a key role in neuronal differentiation : acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach (By similarity).

See full target information ASCL1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MASH1/Achaete-scute homolog 1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MASH1/Achaete-scute homolog 1 antibody (AB235198)

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (AB235198)

Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (AB235198)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise