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AB281184

Anti-MASP1 antibody [EPR22804-121] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal MASP1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

대체 명칭 보기

CRARF, CRARF1, PRSS5, MASP1, Mannan-binding lectin serine protease 1, Complement-activating component of Ra-reactive factor, Mannose-binding lectin-associated serine protease 1, Mannose-binding protein-associated serine protease, Ra-reactive factor serine protease p100, Serine protease 5, MASP-1, RaRF

1 이미지
Sandwich ELISA - Anti-MASP1 antibody [EPR22804-121] - BSA and Azide free (Capture) (AB281184)
  • sELISA

Lab

Sandwich ELISA - Anti-MASP1 antibody [EPR22804-121] - BSA and Azide free (Capture) (AB281184)

Sandwich ELISA of ab253624 with the capture antibody ab281184 dilution at 2 µg/mL and detector antibody ab281034 dilution at 0.5 µg/mL.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22804-121

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB272473

Mouse MASP1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse MASP1 ELISA Kit (AB272473)

  • 0
  • 0
Primary Antibodies

AB305160

Anti-CFHR3 antibody [EPR25759-77] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-CFHR3 antibody [EPR25759-77] - BSA and Azide free (Capture) (AB305160)

  • 0
  • 0
Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

BOND RX™ Validated|KO Validated|RabMAb|Advanced Validation|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

  • 5
  • 133
Primary Antibodies

AB278017

Anti-C4B-d / C4B-b antibody [EPR23364-147] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-C4B-d / C4B-b antibody [EPR23364-147] - BSA and Azide free (Capture) (AB278017)

  • 0
  • 0
Assay Kits

AB253624

Human MASP1 Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human MASP1 Antibody Pair - BSA and Azide free (AB253624)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab281184 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human MASP1. The recommended pair for sandwich ELISA is:
Capture: ab281184, Human MASP1 Capture Antibody (unconjugated)
Detector: ab281034, Human MASP1 Detector Antibody (unconjugated)
The reference range value is 31.25-2000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The MASP1 protein also known as mannan-binding lectin serine protease 1 or MASP-1 is a serine protease with a mass of approximately 76 kDa. It is mainly expressed in the liver and is secreted into the blood. MASP1 is an important component of the lectin pathway of the complement system. This protein is an enzyme that circulates in the blood in an inactive form and becomes active when it binds to pathogen surfaces linked to mannose-binding lectins or ficolins.
Biological function summary

MASP1 plays a vital role in the innate immune system by activating the complement cascade. MASP1 works as part of a complex made up of pattern recognition molecules and other proteases. On binding to pathogens the complex undergoes conformational changes that activate MASP1. This activation leads to the cleavage of downstream substrates including MASP-2 and complement component C4 triggering a series of enzymatic reactions that enhance the pathogen clearance by opsonization and lysis.

Pathways

MASP1 is heavily involved in the lectin pathway one of the three activation pathways of the complement system. It shares functional ties with MASP-2 which continues the cascade after MASP1 activates the pathway. While the lectin pathway runs parallel to the classical pathway it is distinguished by the nature of its initiating molecules that recognize carbohydrate patterns on pathogen surfaces without needing antibodies. MASP1’s role is critical in facilitating the initial steps of this process which eventually leads to the activation of the terminal complement pathway components.

MASP1 shows connections to immune-related conditions such as systemic lupus erythematosus (SLE) and infectious diseases. Its dysregulation or deficiency can impact the complement system leading to immune complex diseases like SLE due to the failure in clearing immune complexes effectively. Furthermore MASP1’s association with MASP-2 also makes it relevant in studies focused on lectin pathway deficiencies that result in increased susceptibility to infections highlighting its significance in maintaining host immunity.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Precursor of a serum protease that activates the complement pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system.. Isoform MASP-1. Serine protease that activates the lectin pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 10946292, PubMed : 11527969, PubMed : 12538697, PubMed : 22691502, PubMed : 22966085, PubMed : 9087411). The lectin complement system is activated following association of lectins, such as MBL2, FCN1, FCN2 or FCN3, to carbohydrates on the pathogen surface (PubMed : 22854970, PubMed : 22966085, PubMed : 23386610, PubMed : 9087411). Following autoproteolytic processing in response to lectin-binding to pathogen carbohydrates, catalyzes cleavage and activation of MASP2, the next component of the lectin complement pathway (PubMed : 22691502, PubMed : 22966085, PubMed : 23386610).. Isoform MASP-3. Serine protease that activates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 20038603, PubMed : 23792966, PubMed : 27535802). The alternative complement pathway acts as an amplification loop that enhances other complement pathways (PubMed : 27535802). Isoform MASP-3 specifically catalyzes cleavage and activation of factor D (CFD), the protease that initiates the alternative complement pathway (PubMed : 20038603, PubMed : 27535802).
See full target information MASP1
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Product promise

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