Anti-Maxi Potassium channel alpha antibody [HL3164] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Maxi Potassium channel alpha antibody [HL3164] - BSA and Azide free (AB327516)

ab327516 detects Maxi Potassium channel alpha protein by immunofluorescent analysis.
Sample : DIV11 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green : Maxi Potassium channel alpha stained by ab327516 diluted at 1 : 250.
Red : Tau, an axon marker, stained by Tau antibody diluted at 1 : 500.
Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-Maxi Potassium channel alpha antibody [HL3164] - BSA and Azide free (AB327516)

Unboiled various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ab327516 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Maxi Potassium channel alpha antibody [HL3164] - BSA and Azide free (ab327516) at 1/1000 dilution

Lane 1:

Unboiled PC-3 whole cell extracts at 30 µg

Lane 2:

Unboiled U87-MG whole cell extracts at 30 µg

Lane 3:

Unboiled Hela whole cell extracts at 30 µg

false

• WB

Supplier Data

Western blot - Anti-Maxi Potassium channel alpha antibody [HL3164] - BSA and Azide free (AB327516)

Boiled and unboiled various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ab327516 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Maxi Potassium channel alpha antibody [HL3164] - BSA and Azide free (ab327516) at 1/1000 dilution

Lane 1:

Boiled mouse brain tissue extracts at 50 µg

Lane 2:

Boiled mouse spleen tissue extracts at 50 µg

Lane 3:

Unboiled mouse brain tissue extracts at 50 µg

Lane 4:

Unboiled mouse spleen tissue extracts at 50 µg

false

• WB

Supplier Data

Western blot - Anti-Maxi Potassium channel alpha antibody [HL3164] - BSA and Azide free (AB327516)

Boiled and unboiled rat tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ab327516 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Maxi Potassium channel alpha antibody [HL3164] - BSA and Azide free (ab327516) at 1/1000 dilution

Lane 1:

Boiled rat brain tissue extracts at 50 µg

Lane 2:

Unboiled rat brain tissue extracts at 50 µg

false