AB240144

Anti-MBD3 antibody [EPR9913] - BSA and Azide free

RabMAb
Recombinant
KO Validated
각 태그의 뜻

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Rabbit Recombinant Monoclonal MBD3 antibody. Carrier free. Suitable for ChIP, WB, ICC/IF, Flow Cyt (Intra), IP and reacts with Mouse, Human, Rat, Cow samples. Cited in 1 publication.

대체 명칭 보기

Methyl-CpG-binding domain protein 3, Methyl-CpG-binding protein MBD3, MBD3

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling MBD3 with purified ab157464 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

Control : PBS only

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

Immunofluorescent analysis of HeLa cells labeling MBD3 with ab157464 at 1/100 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

Flow Cytometry (Intracellular) - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling MBD3 with ab157464 at 1/10 dilution (red) compared to a rabbit IgG negative control (green). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

ChIP

Unknown

ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

Chromatin was prepared from HeLa cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
The ChIP was performed with 25 μg of chromatin, 5 μg of ab157464 (red), and 20 μl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

Primers and probes are located in the first kb of the transcribed region.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

ChIP

Unknown

ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

Chromatin was prepared from NIH/3T3 cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with EGS for 30 minutes, then formaldehyde for 10 minutes.
The ChIP was performed with 25 μg of chromatin, 5 μg of ab157464 (red), and 20 μl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

Primers and probes are located in the first kb of the transcribed region.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab157464).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9913

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Cow, Human

Applications

ICC/IF, Flow Cyt (Intra), IP, WB, ChIP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Cow": { "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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제품 세부 정보

ab240144 is the carrier-free version of ab157464.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The MBD3 protein also known as Methyl-CpG-binding domain protein 3 functions mechanically by binding to methylated DNA which is an essential step in regulating gene expression. It has a molecular mass of approximately 32 kDa. MBD3 is ubiquitously expressed in various tissues demonstrating its role in fundamental cellular processes. The protein does not function alone and often interacts with other proteins as part of gene repression mechanisms.

Biological function summary

The activity of MBD3 impacts chromatin remodeling and gene expression regulation. MBD3 operates as a core component of the nucleosome remodeling deacetylase (NuRD) complex a multi-protein assembly important for transcriptional repression and chromatin structure modification. Its interaction within this complex helps modulate gene expression by altering the accessibility of chromatin and recruiting other modifiers for further regulation.

Pathways

MBD3 plays a significant role in key signaling and epigenetic pathways. It engages in pathways related to chromatin modification and transcription regulation. Its role in the NuRD complex underlines its participation in transcriptional regulation pathways impacting proteins such as HDAC1 and GATA2 which contribute to modifying histone acetylation and influencing gene transcription processes.

Changes in MBD3 expression or function have links to cancer and neurodevelopmental disorders. MBD3 dysregulation associates with tumorigenesis due to its role in maintaining proper gene silencing and expression. Additionally impairment in MBD3 function can relate to intellectual disabilities and developmental delays potentially due to its interaction with epigenetic regulators like MeCP2.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 12124384, PubMed : 16428440, PubMed : 28977666). Acts as transcriptional repressor and plays a role in gene silencing (PubMed : 10947852, PubMed : 18644863). Does not bind to methylated DNA by itself (PubMed : 12124384, PubMed : 16428440). Binds to a lesser degree DNA containing unmethylated CpG dinucleotides (PubMed : 24307175). Recruits histone deacetylases and DNA methyltransferases.

See full target information MBD3

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Bio-protocol 15:e5424 PubMed40873476

2025

Assessing Human Treg Suppression at Single-Cell Resolution Using Mass Cytometry.

Applications

Unspecified application

Species

Unspecified reactive species

Jonas Nørskov Søndergaard,Janyerkye Tulyeu,David Priest,Shimon Sakaguchi,James B Wing

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MBD3 antibody [EPR9913] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

Immunocytochemistry/ Immunofluorescence - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

Flow Cytometry (Intracellular) - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

ChIP - Anti-MBD3 antibody [EPR9913] - BSA and Azide free (AB240144)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise