AB236734

Anti-MC1-R antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal MC1-R antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MC1R aa 1-50.

대체 명칭 보기

MSHR, MC1R, Melanocyte-stimulating hormone receptor, MSH-R, Melanocortin receptor 1, MC1-R

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-MC1-R antibody (AB236734)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MC1-R antibody (AB236734)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained forMC1-R (green) using ab236734 at 1/133 dilution in ICC/IF. Secondary antibody is Alexa Fluor^® 488-congugated Goat Anti-Rabbit IgG(H+L).

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody (AB236734)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody (AB236734)

Paraffin-embedded human melanoma tissue stained for MC1-R using ab236734 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody (AB236734)

Paraffin-embedded human brain tissue stained for MC1-R using ab236734 at 1/400 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MC1R aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q01726

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purity >95%.

보관 버퍼

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The MC1-R also known as the melanocortin 1 receptor is a protein that plays an important role in the regulation of skin and hair pigmentation. It is a G-protein-coupled receptor with a molecular mass of approximately 37 kDa. The receptor is expressed mainly in melanocytes the cells responsible for producing melanin in the skin and hair. Additionally it appears in other cells such as keratinocytes and certain types of immune cells. By binding specific ligands the MC1-R triggers a cascade of intracellular signals that influence melanin production.

Biological function summary

The MC1-R affects pigmentation by increasing the synthesis of eumelanin the dark pigment in the skin and hair while reducing pheomelanin the red or yellow pigment. It functions as part of a complex with other melanocortin receptors working in coordination to modulate the overall pigmentation process in response to environmental cues like ultraviolet radiation. The receptor achieves this by impacting key enzymes involved in melanin production such as tyrosinase.

Pathways

The MC1-R is part of the melanogenesis pathway a critical process for pigmentation protection against UV radiation and development. It interacts with other melanocortin receptors and is activated by melanocyte-stimulating hormones (MSH). The receptor plays a role in the cyclic adenosine monophosphate (cAMP) signaling pathway where it regulates the activity of the protein kinase A (PKA) an essential step in upregulating melanin synthesis. The cAMP pathway also links MC1-R with other related proteins such as CREB which further regulates gene expression.

MC1-R mutations or dysregulations associate with conditions like melanoma and oculocutaneous albinism. In melanoma alterations in MC1-R signaling can lead to increased susceptibility to UV-induced damage as the protective eumelanin is reduced. The receptor also interacts with the protein p53 well known for its role in tumor suppression highlighting its significance in cancer biology. In oculocutaneous albinism MC1-R dysfunction results in decreased melanin production leading to pigmentation deficits and increased risks of skin cancers.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

G protein-coupled receptor that binds melanocyte-stimulating hormones (alpha, beta, and gamma-MSH) and adrenocorticotropic hormone/ACTH, which are peptide products of the POMC precursor protein (PubMed : 11442765, PubMed : 11707265, PubMed : 1325670, PubMed : 1516719, PubMed : 8463333). Upon activation, MC1R couples with the G(s) protein, stimulating adenylate cyclase and activating the cAMP-dependent signaling pathway. This activation promotes melanogenesis, resulting in the production of eumelanin (black/brown) and pheomelanin (red/yellow) in melanocytes (PubMed : 11707265, PubMed : 1325670, PubMed : 16463023, PubMed : 19737927, PubMed : 31097585, PubMed : 34453129). MC1R interacts with G protein-coupled receptor opsin 3/OPN3, which couples to G(i) proteins and inhibits the alpha-MSH-induced cAMP response, thereby reducing melanin synthesis (PubMed : 31097585). Binding to Agouti/ASP precludes alpha-MSH-induced signaling, thereby downregulating melanogenesis (By similarity). Additionally, interaction with MGRN1 displaces the G(s) protein, further suppressing MC1R signaling (PubMed : 19737927).

See full target information MC1R

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 23: PubMed36499015

2022

Fusion Cell Markers in Circulating Tumor Cells from Patients with High-Grade Ovarian Serous Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Paula Carreta Ruano,Andrea Paiva Gadelha Guimarães,Alexcia C Braun,Bianca C T C P Flores,Milena Shizue Tariki,Emne A Abdallah,Jacqueline Aparecida Torres,Diana Noronha Nunes,Bruna Tirapelli,Vladmir C Cordeiro de Lima,Marcello Ferretti Fanelli,Pierre-Emmanuel Colombo,Alexandre André Balieiro Anastácio da Costa,Catherine Alix-Panabières,Ludmilla Thomé Domingos Chinen

Scientific reports 12:18519 PubMed36323745

2022

RhoA and vigilin are candidates for immunohistochemical markers for epithelioid malignant mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Hiratsuka,Takushi Yamamoto,Akihiko Yoshizawa,Shinya Toyokuni,Tatsuaki Tsuruyama

Theranostics 11:2670-2690 PubMed33456566

2021

Visualized podocyte-targeting and focused ultrasound responsive glucocorticoid nano-delivery system against immune-associated nephropathy without glucocorticoid side effect.

Applications

Unspecified application

Species

Unspecified reactive species

Kui Fan,Li Zeng,Jing Guo,Shuqin Xie,Yuan Yu,Jianwei Chen,Jin Cao,Qinyanqiu Xiang,Siliang Zhang,Yuanli Luo,Qingyue Deng,Qin Zhou,Yan Zhao,Lan Hao,Zhigang Wang,Ling Zhong

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MC1-R antibody

Immunocytochemistry/ Immunofluorescence - Anti-MC1-R antibody (AB236734)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody (AB236734)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MC1-R antibody (AB236734)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise