AB50778

Anti-MCAK antibody [2488C3a]

KO Validated
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(2 제품이 사용된 논문 )

Mouse Monoclonal MCAK antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KIF2C.

1 이미지

Lab

Western blot - Anti-MCAK antibody [2488C3a] (AB50778)

Lane 1 : Wild type HAP1 whole cell lysate (20 μg)
Lane 2 : MCAK knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)
Lane 4 : HEK293 whole cell lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab50778 observed at 90 kDa. Red - loading control, ab176560, observed at 50 kDa.

ab50778 was shown to specifically react with MCAK when MCAK knockout samples were used. Wild-type and MCAK knockout samples were subjected to SDS-PAGE. ab50778 and ab176560 (Rabbit anti alpha Tubulin loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with 800CW Goat anti Rabbit and 680CW Goat anti Mouse secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MCAK antibody [2488C3a] (ab50778)

Predicted band size: 81 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

2488C3a

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Fragment Protein within Human KIF2C. The exact immunogen used to generate this antibody is proprietary information.

Q99661

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG depleted (approximately 95%) fetal bovine serum.

보관 버퍼

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The MCAK protein also known as KIF2C (kinesin family member 2C) is a motor protein that makes depolymerization of microtubules. It serves an important role in the regulation of microtubule dynamics during mitosis. MCAK has a molecular mass of approximately 81 kDa. Expression of MCAK is mostly found in actively dividing cells such as those in tissues with high turnover rates including the skin intestinal lining and specific tumors. This protein is critical for the proper formation and functioning of the mitotic spindle during cell division.

Biological function summary

The MCAK protein contributes to accurate chromosome segregation. MCAK performs its functions by binding to microtubule ends and removing tubulin subunits thereby aiding in microtubule disassembly. It functions within the mitotic spindle apparatus and ensures that chromosomes align properly and evenly separate to opposite poles of the dividing cell. This activity is not part of a complex but acts independently to correct improper microtubule attachments.

Pathways

MCAK plays a significant role in mitotic and cell cycle pathways. It coordinates with other proteins like Aurora B kinase which is involved in correcting improper kinetochore-microtubule attachments to maintain chromosome stability during mitosis. MCAK interacts with proteins controlling cell cycle checkpoints overlapping with pathways ensuring cells do not progress with errors in chromosome segregation.

Aberrant regulation of the MCAK protein can lead to chromosomal instability often observed in cancers such as colorectal and breast cancer. This instability can drive tumorigenesis where loss of proper chromosome segregation leads to aneuploidy and cancer progression. In these cancers MCAK may interact with other proteins such as Aurora kinases revealing potential therapeutic targets for intervention in malignancies with defective mitotic processes.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed : 21820309). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed : 19060894). Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (PubMed : 23891108).

See full target information KIF2C

대체 명칭 보기

KNSL6, KIF2C, Kinesin-like protein KIF2C, Kinesin-like protein 6, Mitotic centromere-associated kinesin, MCAK

style

left-column

제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Molecular biology of the cell 28:1223-1237 PubMed28298485

2017

MCAK-mediated regulation of endothelial cell microtubule dynamics is mechanosensitive to myosin-II contractility.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren D'Angelo,Nicole M Myer,Kenneth A Myers

The Journal of cell biology 206:97-112 PubMed25002679

2014

Rac1 and Aurora A regulate MCAK to polarize microtubule growth in migrating endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Braun,Kyvan Dang,Felinah Buslig,Michelle A Baird,Michael W Davidson,Clare M Waterman,Kenneth A Myers

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCAK antibody [2488C3a]

Western blot - Anti-MCAK antibody [2488C3a] (AB50778)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise