Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric)

• WB

Supplier Data

Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) (AB316977)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-mCherry antibody [EPR20579] - Chicken IgY (Chimeric) (ab316977) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T transfected with mCherry expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-preadsorbed-ab7118'>ab7118</a>) at 1/20000 dilution

Observed band size: 27 kDa,36 kDa,35 kDa

false

Exposure time: 10s