AB3723

Anti-MCM2 antibody

5

(1 리뷰)

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(1 출판물)

Goat Polyclonal MCM2 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MCM2 aa 1-50.

2 이미지

Western blot - Anti-MCM2 antibody (AB3723)

WB

Unknown

Western blot - Anti-MCM2 antibody (AB3723)

Sample : Hela cell RIPA extract (60μg).

Antibody : Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000.

Detection : ECL.

Sample : Hela cell RIPA extract (60µg). Antibody : Affinity purified Goat anti-MCM 2 (ab3723) diluted 1 / 4000. Detection : ECL.

All lanes:

Western blot - Anti-MCM2 antibody (ab3723)

Predicted band size: 102 kDa

false

Western blot - Anti-MCM2 antibody (AB3723)

WB

Supplier Data

Western blot - Anti-MCM2 antibody (AB3723)

Detection : Chemiluminescence with an exposed time of 3 minutes.

All lanes:

Western blot - Anti-MCM2 antibody (ab3723) at 0.5 µg/mL

Lane 1:

NIH3T3 cells at 50 µg

Lane 2:

TCMK-1 cells at 50 µg

Lane 3:

4T1 cells at 50 µg

Lane 4:

CT26.WT cells at 50 µg

Predicted band size: 102 kDa

false

주요 정보

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

Immunogen

Synthetic Peptide within Human MCM2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB223403

Alexa Fluor® 647 Anti-MCM2 antibody [EPR4120]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-MCM2 antibody [EPR4120] (AB223403)

0
0

Primary Antibodies

AB133325

Anti-MCM2 antibody [EPR3727]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (AB133325)

0
0

Primary Antibodies

AB133243

Anti-MCM2 (phospho S40) antibody [EPR4170(2)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S40) antibody [EPR4170(2)] (AB133243)

5
2

Primary Antibodies

AB108935

Anti-MCM2 antibody [EPR4120]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)

0
0

Primary Antibodies

AB109270

Anti-MCM2 (phospho S41) antibody [EPR4171]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S41) antibody [EPR4171] (AB109270)

0
0

Primary Antibodies

AB109133

Anti-MCM2 (phospho S53) antibody [EP4120]

20ul selling size|RabMAb|Recombinant

5
4

Primary Antibodies

AB109271

Anti-MCM2 (phospho S108) antibody [EPR4121]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (AB109271)

0
0

보완 제품

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

5
24

Primary Antibodies

AB109271

Anti-MCM2 (phospho S108) antibody [EPR4121]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (AB109271)

0
0

Primary Antibodies

AB92552

Anti-PCNA antibody [EPR3821]

RabMAb|Lab Essentials|20ul selling size|Recombinant

Flow Cytometry (Intracellular) - Anti-PCNA antibody [EPR3821] (AB92552)

5
12

Primary Antibodies

AB6326

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

Flow Cytometry (Intracellular) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (AB6326)

5
104

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MCM2 or Minichromosome Maintenance Complex Component 2 is a protein involved in the initiation of DNA replication. It plays a central role in the formation of the pre-replication complex which is necessary for DNA unwinding and replication fork progression. The molecular weight of MCM2 is approximately 100 kDa. It is expressed ubiquitously in proliferating cells as its function is necessary for DNA replication. Researchers often use terms like MCM-2 MC M2 or PMC M2 when discussing varying aspects of its function and interaction with other proteins in scientific literature.

Biological function summary

MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.

Pathways

MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.

MCM2 is often associated with cancer and some types of genomic instability disorders. Its overexpression can lead to uncontrolled cell proliferation a hallmark of cancer. MCM2 expression levels can be a marker in distinguishing certain tumors acting as a potential therapeutic target. The protein's interaction with components like cyclin-dependent kinases (CDKs) can influence pathways leading to malignant transformation and suggests its involvement in tumorigenesis and potential as a target for anticuerpos or antibodies in disease treatment.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for the entry in S phase and for cell division (PubMed : 8175912). Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed : 26196677).

See full target information MCM2

대체 명칭 보기

BM28, CCNL1, CDCL1, KIAA0030, MCM2, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Journal of occupational health 56:186-96 PubMed24598051

2014

Formation of the nitrative DNA lesion 8-nitroguanine is associated with asbestos contents in human lung tissues: a pilot study.

Applications

Unspecified application

Species

Unspecified reactive species

Yusuke Hiraku,Kiyoshi Sakai,Eiji Shibata,Michihiro Kamijima,Naomi Hisanaga,Ning Ma,Shosuke Kawanishi,Mariko Murata

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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