AB248477

Anti-MCM2 antibody [EPR3727] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MCM2 antibody. Carrier free. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

BM28, CCNL1, CDCL1, KIAA0030, MCM2, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28

12 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab133325 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling MCM2 with ab133325 at 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling MCM2 with ab133325 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Urinary bladder transitional carcinoma tissue using ab133325 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling MCM2 with unpurified ab133325 at 1/200 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human heart tissue using ab133325 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab133325 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling MCM2 with ab133325 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133325 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human Clear cell carcinoma of kidney tissue using ab133325 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.

MCM2 was immunoprecipitated from Jurkat (Human T cell leukemia cell line from peripheral blood) with ab133325 at 1/60 dilution.

Exposue time : 10 second.

All lanes:

Immunoprecipitation - Anti-MCM2 antibody [EPR3727] (<a href='/ko/products/primary-antibodies/mcm2-antibody-epr3727-ab133325'>ab133325</a>)

Predicted band size: 102 kDa

false

Unknown

Western blot - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

This data was developed using ab133325, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MCM2 antibody [EPR3727] (<a href='/ko/products/primary-antibodies/mcm2-antibody-epr3727-ab133325'>ab133325</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

MCF-7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 102 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3727

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, ICC/IF, Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab248477 is the carrier-free version of ab133325.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MCM2 or Minichromosome Maintenance Complex Component 2 is a protein involved in the initiation of DNA replication. It plays a central role in the formation of the pre-replication complex which is necessary for DNA unwinding and replication fork progression. The molecular weight of MCM2 is approximately 100 kDa. It is expressed ubiquitously in proliferating cells as its function is necessary for DNA replication. Researchers often use terms like MCM-2 MC M2 or PMC M2 when discussing varying aspects of its function and interaction with other proteins in scientific literature.

Biological function summary

MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.

Pathways

MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.

MCM2 is often associated with cancer and some types of genomic instability disorders. Its overexpression can lead to uncontrolled cell proliferation a hallmark of cancer. MCM2 expression levels can be a marker in distinguishing certain tumors acting as a potential therapeutic target. The protein's interaction with components like cyclin-dependent kinases (CDKs) can influence pathways leading to malignant transformation and suggests its involvement in tumorigenesis and potential as a target for anticuerpos or antibodies in disease treatment.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for the entry in S phase and for cell division (PubMed : 8175912). Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed : 26196677).

See full target information MCM2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncology letters 24:457 PubMed36380881

2022

Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Ji Cao,Wan-Li Ge,Ling-Dong Meng,Qun Chen,Yi Miao,Kui-Rong Jiang,Jing-Jing Zhang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCM2 antibody [EPR3727] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Flow Cytometry (Intracellular) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Immunoprecipitation - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

Western blot - Anti-MCM2 antibody [EPR3727] - BSA and Azide free (AB248477)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise