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AB70371

Anti-MCM2 (phospho S40 + S41) antibody

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(5 제품이 사용된 논문 )

Rabbit Polyclonal MCM2 phospho S40 + S41 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MCM2 pS40 + S41.
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Flow Cytometry (Intracellular) - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)

ab70371 at 0.1μg/ml staining MCM2 (phospho S40 + S41) by Flow Cytometery. 1x106 jurkat cells were fixed in 1.5% PFA and permeabilized in 90% MeOH. DNA content was simultaneously analyzed via PI stain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right) tissues labelling MCM2 (phospho S40 + S41) with ab70371 at 1/200 (1µg/ml). Detection : DAB.

Western blot - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)
  • WB

Unknown

Western blot - Anti-MCM2 (phospho S40 + S41) antibody (AB70371)

The bottom panel shows total MCM2 staining; the blot was stripped and then blotted with rabbit anti-total MCM2 antibody at 0.1 µg/ml. Detection : Chemiluminescence with exposure times of 30 seconds for upper and lower panel.

All lanes:

Western blot - Anti-MCM2 (phospho S40 + S41) antibody (ab70371) at 0.1 µg/mL

Lane 1:

Whole cell lysate from asynchronous 293T cells at 50 µg

Lane 2:

Whole cell lysate from asynchronous 293T cells treated with a phosphatase at 50 µg

Predicted band size: 102 kDa

Observed band size: 117 kDa,60 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

Synthetic Peptide within Human MCM2 pS40 + S41. The exact immunogen used to generate this antibody is proprietary information.

P49736

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대체품

Primary Antibodies

AB223403

Alexa Fluor® 647 Anti-MCM2 antibody [EPR4120]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 647 Anti-MCM2 antibody [EPR4120] (AB223403)

  • 0
  • 0
Primary Antibodies

AB133325

Anti-MCM2 antibody [EPR3727]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody [EPR3727] (AB133325)

  • 0
  • 0
Primary Antibodies

AB133243

Anti-MCM2 (phospho S40) antibody [EPR4170(2)]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S40) antibody [EPR4170(2)] (AB133243)

  • 5
  • 2
Primary Antibodies

AB108935

Anti-MCM2 antibody [EPR4120]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)

  • 0
  • 0
Primary Antibodies

AB109270

Anti-MCM2 (phospho S41) antibody [EPR4171]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S41) antibody [EPR4171] (AB109270)

  • 0
  • 0
Primary Antibodies

AB109133

Anti-MCM2 (phospho S53) antibody [EP4120]

20ul selling size|RabMAb|Recombinant

  • 5
  • 4
Primary Antibodies

AB109271

Anti-MCM2 (phospho S108) antibody [EPR4121]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 (phospho S108) antibody [EPR4121] (AB109271)

  • 0
  • 0

보완 제품

Primary Antibodies

AB108935

Anti-MCM2 antibody [EPR4120]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MCM2 antibody [EPR4120] (AB108935)

  • 0
  • 0
Primary Antibodies

AB109270

Anti-MCM2 (phospho S41) antibody [EPR4171]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S41) antibody [EPR4171] (AB109270)

  • 0
  • 0
Primary Antibodies

AB109133

Anti-MCM2 (phospho S53) antibody [EP4120]

20ul selling size|RabMAb|Recombinant

  • 5
  • 4
Primary Antibodies

AB109459

Anti-MCM2 (phospho S27) antibody [EPR4119]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 (phospho S27) antibody [EPR4119] (AB109459)

  • 0
  • 0
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Reactivity 정보

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Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle|Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MCM2 or Minichromosome Maintenance Complex Component 2 is a protein involved in the initiation of DNA replication. It plays a central role in the formation of the pre-replication complex which is necessary for DNA unwinding and replication fork progression. The molecular weight of MCM2 is approximately 100 kDa. It is expressed ubiquitously in proliferating cells as its function is necessary for DNA replication. Researchers often use terms like MCM-2 MC M2 or PMC M2 when discussing varying aspects of its function and interaction with other proteins in scientific literature.
Biological function summary

MCM2 is part of the heterohexameric MCM complex including other proteins like MCM3 to MCM7. This complex serves as a helicase unwinding DNA to allow replication machinery access to the template strand. The activity of the MCM complex is tightly regulated to ensure that every section of genomic DNA is replicated once per cell cycle. Its proper function ensures genetic information replication accuracy and contributes to genome stability.

Pathways

MCM2 participates in essential biological processes such as the DNA replication pathway and the cell cycle regulatory pathway. The presence of MCM2 is critical for the G1/S transition phase of the cell cycle. It interacts with other proteins including CDC45 and GINS forming the active CMG helicase complex. This complex is pivotal in orchestrating the replication machinery and signaling pathway responses to ensure appropriate replication origin firing and accurate DNA synthesis.

MCM2 is often associated with cancer and some types of genomic instability disorders. Its overexpression can lead to uncontrolled cell proliferation a hallmark of cancer. MCM2 expression levels can be a marker in distinguishing certain tumors acting as a potential therapeutic target. The protein's interaction with components like cyclin-dependent kinases (CDKs) can influence pathways leading to malignant transformation and suggests its involvement in tumorigenesis and potential as a target for anticuerpos or antibodies in disease treatment.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for the entry in S phase and for cell division (PubMed : 8175912). Plays a role in terminally differentiated hair cells development of the cochlea and induces cells apoptosis (PubMed : 26196677).
See full target information MCM2 pS40 + S41

대체 명칭 보기

BM28, CCNL1, CDCL1, KIAA0030, MCM2, DNA replication licensing factor MCM2, Minichromosome maintenance protein 2 homolog, Nuclear protein BM28
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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomedicines 10: PubMed36009559

2022

Dbf4-Cdc7 (DDK) Inhibitor PHA-767491 Displays Potent Anti-Proliferative Effects via Crosstalk with the CDK2-RB-E2F Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tekle Pauzaite,James Tollitt,Betul Sopaci,Louise Caprani,Olivia Iwanowytsch,Urvi Thacker,John G Hardy,Sarah L Allinson,Nikki A Copeland

Cell death discovery 8:85 PubMed35220396

2022

CDC7 kinase (DDK) inhibition disrupts DNA replication leading to mitotic catastrophe in Ewing sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jeffrey C Martin,Jennie R Sims,Ajay Gupta,Tamara J Hagoel,Lingqiu Gao,Miranda L Lynch,Anna Woloszynska,Thomas Melendy,Jeremy F Kane,Joseph Kuechle,Joyce E Ohm

Journal of experimental & clinical cancer research : CR 40:82 PubMed33648524

2021

Splicing factors control triple-negative breast cancer cell mitosis through SUN2 interaction and sororin intron retention.

Applications

Unspecified application

Species

Unspecified reactive species

Esmee Koedoot,Eline van Steijn,Marjolein Vermeer,Román González-Prieto,Alfred C O Vertegaal,John W M Martens,Sylvia E Le Dévédec,Bob van de Water

Breast cancer research : BCR 21:77 PubMed31262335

2019

A kinase inhibitor screen identifies a dual cdc7/CDK9 inhibitor to sensitise triple-negative breast cancer to EGFR-targeted therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ronan P McLaughlin,Jichao He,Vera E van der Noord,Jevin Redel,John A Foekens,John W M Martens,Marcel Smid,Yinghui Zhang,Bob van de Water

Cancer cell international 16:88 PubMed27891063

2016

Cell division cycle 7-kinase inhibitor PHA-767491 hydrochloride suppresses glioblastoma growth and invasiveness.

Applications

WB

Species

Human

Zubeyde Erbayraktar,Begum Alural,Resat Serhat Erbayraktar,Erdogan Pekcan Erkan
제품이 사용된 논문 모두 보기
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Product promise

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