AB4459

Anti-MCM4 antibody

Name: Anti-MCM4 antibody
Brand: Abcam Limited
SKU: AB4459
Rating: 5 (1 reviews)

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(36 제품이 사용된 논문 )

Rabbit Polyclonal MCM4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Xenopus laevis samples. Cited in 36 publications. Immunogen corresponding to Synthetic Peptide within Human MCM4 aa 750-800.

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB4459)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB4459)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM4 with ab4459 at 1/1000 (1µg/ml). Detection : DAB.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB4459)

IHC image of MCM4 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4459, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (AB4459)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (AB4459)

ICC/IF image of ab4459 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4459, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Unknown

Western blot - Anti-MCM4 antibody (AB4459)

This image is an edited version of an image received courtesy of an Abreview submitted by Jason DeHart on 22 August 2005. We do not have any further information relating to this image.

All lanes:

Western blot - Anti-MCM4 antibody (ab4459) at 1/2000 dilution

All lanes:

HeLa nuclear cell lysate at 20 µg

Secondary

All lanes:

Goat anti rabbit HRP at 1/5000 dilution

Predicted band size: 97 kDa

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Unknown

Western blot - Anti-MCM4 antibody (AB4459)

HeLa cell RIPA extract incubated with 0.2 μg/ml of ab4459 for 1 hour. HeLa cell RIPA extract incubated with 0.2 µg/ml of ab4459 for 1 hour.

All lanes:

Western blot - Anti-MCM4 antibody (ab4459)

Predicted band size: 97 kDa

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CiteAb

Western blot - Anti-MCM4 antibody (AB4459)

Western Blotting using Anti-MCM4 antibody, ab4459. Publication image from Kessler, B. M. et al., 2015, Nucleic Acids Res, 25800737. Legend direct from paper.

XRCC1 knockdown activates a DNA damage response and delays cell cycle progression. (A and D) Western blot analysis of the indicated proteins upon XRCC1 KD.α-Tubulin served as a loading control. (B) Quantifications of p21 protein levels as seen in (A). Values are mean ± SD from four independent experiments, normalized to the loading control. The P-value was calculated using Student's t-test. (C) FACS analysis of cell-cycle distribution after XRCC1 KD. Shown is the percentage of cells in the respective cell cycle phases after control treatment or XRCC1 KD. Values are mean ± SD from three independent experiments. The P-value was calculated using Student's two-tailed t-test. (E) Quantification of protein levels as seen in (D). Values are mean ± SD from four independent experiments, normalized to the loading control. The P-value was calculated using Student's t-test.

false

CiteAb

Western blot - Anti-MCM4 antibody (AB4459)

Western Blotting using Anti-MCM4 antibody, ab4459. Publication image from Raynaud, P. et al., 2017, Nat Commun, 28785014. Legend direct from paper.

Ensa KD increases S-phase length by decreasing fork number without affecting origin licensing. a Diagram depicting IdU (red)–CldU (green) double pulse analysis by DNA combing. b Representative image of a triple-stained DNA fibre : IdU (red)–CldU (green)–DNA (blue). c Fork velocity (Kb/min) and d global instant fork density (GIFD) values (fork per Mb) are represented. Mean ± standard deviation and p-value obtained by using two-tailed unpaired Student’s t-tests, are represented. Data pooled from four biological replicates. e Diagram representing the experiment in which HeLa cells were blocked in G1–S phase by thymidine for 24 h and subsequently released with fresh medium containing nocodazole (50 ng/ml) for a supplementary period of 16 h before shakeoff. Four hours later, released cells were transfected with siSC or siEnsa, and after 1 h, supplemented with fresh medium containing thymidine for an additional 18 h incubation. Cells were then released and recovered at the indicated times for FACS analysis. f FACS profiles of SC (red) and Ensa (blue) siRNA-treated cells at different time points after release are shown. g HeLa and U2OS cells were transfected with scramble (siSC) or Ensa (siEnsa) siRNAs and 48 h later processed for chromatin isolation as reported in Methods section. Cytosolic (S2) and nuclear (S3) soluble fractions as well as chromatin fraction (P3) were then used for western blot analysis to determine Treslin and MCM 2, 3 and 4 levels. h As for g, except that MCM 5 and 7, histone H3 and β-tubulin levels were checked. IOD, inter-origin distances; Ori, replication origin

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human, Xenopus laevis

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human MCM4 aa 750-800. The exact immunogen used to generate this antibody is proprietary information.

P33991

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Drosophila melanogaster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Opossum": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MCM4 or Minichromosome Maintenance Complex Component 4 is a part of the MCM2-7 complex which is essential for the initiation and progression of DNA replication. It has a molecular mass of approximately 95 kDa. MCM4 functions as a DNA helicase unwinding DNA strands to allow replication forks to progress. This protein is expressed in various tissues with higher levels detected in dividing cells indicating its role in cell proliferation.

Biological function summary

MCM4 is a critical player in cell cycle regulation as it participates in the formation of the pre-replicative complex during the G1 phase of the cell cycle. Its function becomes evident as it interacts within the MCM2-7 hexameric complex important for helicase activity and stability. This complex prevents DNA re-replication within a single cell cycle ensuring the maintenance of genomic integrity.

Pathways

Several cellular mechanisms engage MCM4 particularly the DNA replication and cell cycle pathways. MCM4 associates with proteins like CDC45 and GINS within the CMG complex facilitating the activation and elongation phases of DNA replication. This relationship places MCM4 at the heart of orchestrating proper DNA synthesis and mitotic entry.

Researchers have linked MCM4 abnormalities to cancer progression and genomic instability. Overexpression or mutations in MCM4 disrupts normal replication processes heightening the risk for various cancers including breast and ovarian cancer. It also demonstrates interaction with the tumor suppressor protein p53 suggesting its involvement in processes that underpin malignancies linked to failed checkpoint control.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 16899510, PubMed : 25661590, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232, PubMed : 9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 16899510, PubMed : 25661590, PubMed : 32453425, PubMed : 9305914).

See full target information MCM4

대체 명칭 보기

CDC21, MCM4, DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21

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제품이 사용된 논문 (36)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature 627:204-211 PubMed38383787

2024

Targeted protein degradation via intramolecular bivalent glues.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver Hsia,Matthias Hinterndorfer,Angus D Cowan,Kentaro Iso,Tasuku Ishida,Ramasubramanian Sundaramoorthy,Mark A Nakasone,Hana Imrichova,Caroline Schätz,Andrea Rukavina,Koraljka Husnjak,Martin Wegner,Alejandro Correa-Sáez,Conner Craigon,Ryan Casement,Chiara Maniaci,Andrea Testa,Manuel Kaulich,Ivan Dikic,Georg E Winter,Alessio Ciulli

EMBO reports 25:876-901 PubMed38177925

2024

FANCJ DNA helicase is recruited to the replisome by AND-1 to ensure genome stability.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Boavida,Luisa Mr Napolitano,Diana Santos,Giuseppe Cortone,Nanda K Jegadesan,Silvia Onesti,Dana Branzei,Francesca M Pisani

Nature communications 14:8049 PubMed38081811

2023

RIF1 regulates early replication timing in murine B cells.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Malzl,Mihaela Peycheva,Ali Rahjouei,Stefano Gnan,Kyle N Klein,Mariia Nazarova,Ursula E Schoeberl,David M Gilbert,Sara C B Buonomo,Michela Di Virgilio,Tobias Neumann,Rushad Pavri

The EMBO journal 42:e114131 PubMed37458194

2023

Novel role of DONSON in CMG helicase assembly during vertebrate DNA replication initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshitami Hashimoto,Kota Sadano,Nene Miyata,Haruka Ito,Hirofumi Tanaka

Nature 606:197-203 PubMed35585235

2022

MCM complexes are barriers that restrict cohesin-mediated loop extrusion.

Applications

Unspecified application

Species

Unspecified reactive species

Bart J H Dequeker,Matthias J Scherr,Hugo B Brandão,Johanna Gassler,Sean Powell,Imre Gaspar,Ilya M Flyamer,Aleksandar Lalic,Wen Tang,Roman Stocsits,Iain F Davidson,Jan-Michael Peters,Karl E Duderstadt,Leonid A Mirny,Kikuë Tachibana

eLife 11: PubMed35438632

2022

MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichiro Saito,Venny Santosa,Kei-Ichiro Ishiguro,Masato T Kanemaki

Life science alliance 5: PubMed35292537

2022

Mre11 exonuclease activity promotes irreversible mitotic progression under replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshitami Hashimoto,Hirofumi Tanaka

Current protocols 1:e219 PubMed34370399

2021

Targeted Protein Depletion Using the Auxin-Inducible Degron 2 (AID2) System.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichiro Saito,Masato T Kanemaki

Scientific reports 11:3761 PubMed33580144

2021

Arsenic hexoxide has differential effects on cell proliferation and genome-wide gene expression in human primary mammary epithelial and MCF7 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Donguk Kim,Na Yeon Park,Keunsoo Kang,Stuart K Calderwood,Dong-Hyung Cho,Ill Ju Bae,Heeyoun Bunch

OncoTargets and therapy 13:12015-12025 PubMed33244243

2020

Huaier Suppresses the Hepatocellular Carcinoma Cell Cycle by Regulating Minichromosome Maintenance Proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjie Niu,Liang Shan,Han Gao,Congcong Zhang,Zijun Qian,Zhixian Wang,Xin Xu,Xiao Zhang,Jiayi Wang,Lifang Ma,Liyun Chen,Yongchun Yu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCM4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB4459)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB4459)

Immunocytochemistry/ Immunofluorescence - Anti-MCM4 antibody (AB4459)

Western blot - Anti-MCM4 antibody (AB4459)

Western blot - Anti-MCM4 antibody (AB4459)

Western blot - Anti-MCM4 antibody (AB4459)

Western blot - Anti-MCM4 antibody (AB4459)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (36)

Product promise