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AB74014

Anti-MCM4 (phospho S54) antibody

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(1 출판물)

Rabbit Polyclonal MCM4 phospho S54 antibody. Suitable for WB and reacts with African green monkey samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human MCM4 pS54.
1 이미지
Western blot - Anti-MCM4 (phospho S54) antibody (AB74014)
  • WB

Unknown

Western blot - Anti-MCM4 (phospho S54) antibody (AB74014)

All lanes:

Western blot - Anti-MCM4 (phospho S54) antibody (ab74014) at 1/500 dilution

Lane 1:

Extracts from COS-7 cells treated with nocodazole (1uM, 16hours) at 5 µg

Lane 2:

Extracts from COS-7 cells treated with nocodazole (1uM, 16hours) at 5 µg with immunising phosphopeptide

Predicted band size: 97 kDa

Observed band size: 20 kDa,88 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

African green monkey

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MCM4 pS54. The exact immunogen used to generate this antibody is proprietary information.

P33991

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대체품

Primary Antibodies

AB4459

Anti-MCM4 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB4459)

  • 5
  • 1
Primary Antibodies

AB3728

Anti-MCM4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB3728)

  • 0
  • 0
Primary Antibodies

AB154315

Anti-MCM4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB154315)

  • 1
  • 1
Primary Antibodies

AB124836

Anti-MCM4 antibody [EPR5245]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MCM4 antibody [EPR5245] (AB124836)

  • 0
  • 0

보완 제품

Primary Antibodies

AB4459

Anti-MCM4 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM4 antibody (AB4459)

  • 5
  • 1
Primary Antibodies

AB75975

Anti-MCM5 antibody [EP2683Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MCM5 antibody [EP2683Y] (AB75975)

  • 5
  • 1
Primary Antibodies

AB191857

Anti-SREBP1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SREBP1 antibody (AB191857)

  • 0
  • 0
Primary Antibodies

AB30682

Anti-SREBP2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SREBP2 antibody (AB30682)

  • 4
  • 12
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab74014 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
보관 버퍼
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MCM4 or Minichromosome Maintenance Complex Component 4 is a part of the MCM2-7 complex which is essential for the initiation and progression of DNA replication. It has a molecular mass of approximately 95 kDa. MCM4 functions as a DNA helicase unwinding DNA strands to allow replication forks to progress. This protein is expressed in various tissues with higher levels detected in dividing cells indicating its role in cell proliferation.
Biological function summary

MCM4 is a critical player in cell cycle regulation as it participates in the formation of the pre-replicative complex during the G1 phase of the cell cycle. Its function becomes evident as it interacts within the MCM2-7 hexameric complex important for helicase activity and stability. This complex prevents DNA re-replication within a single cell cycle ensuring the maintenance of genomic integrity.

Pathways

Several cellular mechanisms engage MCM4 particularly the DNA replication and cell cycle pathways. MCM4 associates with proteins like CDC45 and GINS within the CMG complex facilitating the activation and elongation phases of DNA replication. This relationship places MCM4 at the heart of orchestrating proper DNA synthesis and mitotic entry.

Researchers have linked MCM4 abnormalities to cancer progression and genomic instability. Overexpression or mutations in MCM4 disrupts normal replication processes heightening the risk for various cancers including breast and ovarian cancer. It also demonstrates interaction with the tumor suppressor protein p53 suggesting its involvement in processes that underpin malignancies linked to failed checkpoint control.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 16899510, PubMed : 25661590, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232, PubMed : 9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 16899510, PubMed : 25661590, PubMed : 32453425, PubMed : 9305914).
See full target information MCM4 pS54

대체 명칭 보기

CDC21, MCM4, DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer biology & therapy 26:2471631 PubMed39992057

2025

CXCR4 confers stemness and radioresistance in chordoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chan-Woong Jung,Jeong-Yub Kim,Myung-Jin Park
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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