AB4458

Anti-MCM6 antibody

Name: Anti-MCM6 antibody
Brand: Abcam Limited
SKU: AB4458
Rating: 4 (1 reviews)

BOND RX™ Validated
각 태그의 뜻

(1 리뷰)

(5 제품이 사용된 논문 )

Rabbit Polyclonal MCM6 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human MCM6.

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (AB4458)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (AB4458)

IHC image of ab4458 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4458, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (AB4458)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling MCM6 with ab4458 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Western blot - Anti-MCM6 antibody (AB4458)

All lanes:

Western blot - Anti-MCM6 antibody (ab4458) at 0.2 µg/mL

All lanes:

RIPA extract from HeLa whole cell lysate at 50 µg

Predicted band size: 93 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MCM6. The exact immunogen used to generate this antibody is proprietary information.

Q14566

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The target known as MCM6 or Minichromosome Maintenance Complex Component 6 is a part of a group of proteins providing essential functions during DNA replication initiation. MCM6 is a part of the hexameric MCM2-7 complex which operates as a helicase that unwinds DNA to facilitate fork progression. This protein has an approximate molecular weight of 92 kDa. MCM6 expresses in dividing cells across different tissues particularly in cells with high proliferative rates like those in the bone marrow and gastrointestinal tract.

Biological function summary

The functions of MCM6 contribute to the precise control of DNA replication and overall cell cycle regulation. The protein forms a part of the MCM2-7 complex playing a significant role in the S phase to ensure DNA replication occurs at the right time. Additionally the MCM complex acts as a primary licensing factor for replication origins preventing re-replication. MCM6 also interacts with other proteins like CDC45 and GINS to form the CMG complex a critical component for replication fork stability and duplication.

Pathways

MCM6 contributes significantly within the DNA replication and cell cycle pathways. As a component of the MCM2-7 complex it supports the unwinding of DNA which is a prerequisite for replication to initiate. MCM6 is related to proteins like CDC6 and ORC which are integral to the assembly of the pre-replication complex facilitating the initiation of DNA synthesis. This integration into core cellular mechanisms highlights its importance in genomic stability.

Mutations or deregulation of MCM6 have links to cancerous growths and genomic instability disorders. The aberrant expression of MCM6 is often observed in cancers such as colorectal and breast cancer where it contributes to unchecked cellular proliferation. MCM6 correlates with oncogenic proteins like MYC which can amplify abnormal cell cycle progression. Understanding the mechanistic contributions of MCM6 in these conditions assists in developing therapeutic strategies targeting these pathways.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 16899510, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232, PubMed : 9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425).

See full target information MCM6

대체 명칭 보기

DNA replication licensing factor MCM6, p105MCM, MCM6

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of Cancer 10:4488-4498 PubMed31528213

2019

CDK5RAP3 Inhibits the Translocation of MCM6 to Influence the Prognosis in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qi-Yue Chen,Li-Chao Liu,Jia-Bin Wang,Jian-Wei Xie,Jian-Xian Lin,Jun Lu,Long-Long Cao,Mi Lin,Ru-Hong Tu,Chang-Ming Huang,Ping Li,Chao-Hui Zheng

Neoplasia (New York, N.Y.) 16:694-709 PubMed25246271

2014

Deregulation of the replisome factor MCMBP prompts oncogenesis in colorectal carcinomas through chromosomal instability.

Applications

Unspecified application

Species

Human

Mauricio Quimbaya,Eric Raspé,Geertrui Denecker,Bram De Craene,Ria Roelandt,Wim Declercq,Xavier Sagaert,Lieven De Veylder,Geert Berx

Biochimica et biophysica acta 1843:2432-7 PubMed25003315

2014

The MUC1 mucin regulates the tumorigenic properties of human esophageal adenocarcinomatous cells.

Applications

Unspecified application

Species

Unspecified reactive species

Caroline Gronnier,Emilie Bruyère,Fatima Lahdaoui,Nicolas Jonckheere,Michaël Perrais,Emmanuelle Leteurtre,Guillaume Piessen,Christophe Mariette,Isabelle Van Seuningen

Mammalian genome : official journal of the Interna 22:506-17 PubMed21611832

2011

A reduction of licensed origins reveals strain-specific replication dynamics in mice.

Applications

Species

Mouse

Tsuyoshi Kawabata,Satoru Yamaguchi,Tavanna Buske,Spencer W Luebben,Marsha Wallace,Ilze Matise,John C Schimenti,Naoko Shima

PLoS genetics 6:e1000817 PubMed20090939

2010

The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair.

Applications

Species

Human

Naoki Takahashi,Mauricio Quimbaya,Veit Schubert,Tim Lammens,Klaas Vandepoele,Ingo Schubert,Minami Matsui,Dirk Inzé,Geert Berx,Lieven De Veylder

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCM6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (AB4458)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM6 antibody (AB4458)

Western blot - Anti-MCM6 antibody (AB4458)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise