AB96849

Anti-MCM7/PRL antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal MCM7/PRL antibody. Suitable for WB, IP, ChIP, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCM7 aa 1 to C-terminus.

4 이미지

Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody (AB96849)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody (AB96849)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells using ab96849 at 1/200 dilution (1).Merged with DNA probe (2).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody (AB96849)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody (AB96849)

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft using ab96849 at 1/100 dilution.

Supplier Data

Immunoprecipitation - Anti-MCM7/PRL antibody (AB96849)

MCM7 was immunoprecipitated from 40 μg of HeLa whole cell lysate with ab96849 at 1/1000 dilution. EasyBlot anti-rabbit IgG, was used as secondary antibody.
Lane 1 : 40 μg HeLa whole cell extract (Input).
Lane 2 : Control with 4 μg of preimmune Rabbit IgG.
Lane 3 : Immunoprecipitation of MCM7 protein by 4 μg MCM7 antibody [N2C2], Internal.

7.5 % SDS-PAGE

All lanes:

Immunoprecipitation - Anti-MCM7/PRL antibody (ab96849)

Predicted band size: 81 kDa

false

ChIP

Supplier Data

ChIP - Anti-MCM7/PRL antibody (AB96849)

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-MCM7 antibody. The precipitated DNA was detected by PCR with primer set targeting to Myc promoter.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IP, IHC-P, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human MCM7 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P33993

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The MCM7 (Minichromosome Maintenance Complex Component 7) also known as PRL serves an important mechanical role in DNA replication. It is part of the MCM2-7 complex a helicase essential for the initiation and elongation phases of DNA replication. MCM7 has a molecular mass of approximately 81 kDa and is found expressed in the nucleus of proliferating cells. It is essential for unwinding the DNA helix preparing it for replication.

Biological function summary

MCM7 is involved in maintaining genomic stability and ensuring accurate DNA replication. As part of the larger MCM2-7 hexameric complex it collaborates with other members to form the core component of the pre-replicative complex (pre-RC). By loading onto replication origins MCM7 helps license the DNA for duplication. This function contributes to cell cycle progression particularly the S phase where DNA synthesis occurs.

Pathways

MCM7 plays an important role in DNA replication-related signaling pathways. It functions within the cell cycle and DNA replication pathways interacting closely with other essential proteins like CDC45 and GINS complex. These partnerships facilitate the transition from the G1 to the S phase triggering the replication machinery's activation and ensuring cell division processes continue smoothly.

MCM7 has been associated with various cancers including breast and prostate cancer. Aberrant expression or dysregulation of MCM7 contributes to uncontrolled cell proliferation linking it to tumorigenesis. Also MCM7 interacts with proteins such as cyclin D1 in certain oncogenic pathways emphasizing its role in the progression of cancerous conditions. Understanding these interactions offers insights into potential therapeutic targets for cancer treatment.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Acts as a component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 25661590, PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232, PubMed : 9305914). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed : 32453425). Required for S-phase checkpoint activation upon UV-induced damage.

See full target information MCM7

대체 명칭 보기

CDC47, MCM2, MCM7, DNA replication licensing factor MCM7, CDC47 homolog, P1.1-MCM3

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Tohoku journal of experimental medicine : PubMed40738669

2025

Histone Lactylation Promotes Proliferation and Migration of TNBC Cells via Upregulating MCM7 Transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Xiao,Chaoxian Wang,Sili Jin,Ziran Zhang,Xingxia Yang,Guangfa Xia

Cell reports 7:575-587 PubMed24726359

2014

The RBBP6/ZBTB38/MCM10 axis regulates DNA replication and common fragile site stability.

Applications

Species

Unspecified reactive species

Benoit Miotto,Moredreck Chibi,Ping Xie,Stéphane Koundrioukoff,Hanlie Moolman-Smook,David Pugh,Michelle Debatisse,Fuchu He,Lingqiang Zhang,Pierre-Antoine Defossez

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MCM7/PRL antibody

Immunocytochemistry/ Immunofluorescence - Anti-MCM7/PRL antibody (AB96849)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM7/PRL antibody (AB96849)

Immunoprecipitation - Anti-MCM7/PRL antibody (AB96849)

ChIP - Anti-MCM7/PRL antibody (AB96849)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise