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AB9858

Anti-MCP1 antibody [2.2-4H5-1A11]

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(1 리뷰)

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(6 제품이 사용된 논문 )

Mouse Monoclonal MCP1 antibody. Suitable for ELISA, WB, sELISA, IHC-P and reacts with Recombinant full length protein - Human, Recombinant fragment - Human, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCL2.
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Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)

In a sandwich ELISA (assuming 100 μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 100 pg/mL of Recombinant Human MCP-1 when used with Biotin Anti-MCP1 antibody (ab271250) as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.

Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)
  • sELISA

Unknown

Sandwich ELISA - Anti-MCP1 antibody [2.2-4H5-1A11] (AB9858)

Standard curve for MCP1 (Analyte : ab73866); dilution range 1pg/ml to 1μg/ml using Capture Antibody Mouse monoclonal to MCP1 (ab9858) at 1μg/ml and Detector Antibody Rabbit polyclonal to MCP1 (ab9669) at 0.5μg/ml.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

2.2-4H5-1A11

Isotype

IgG1

Light chain 타입

kappa

Carrier free

No

Reacts with

Human

Applications

ELISA, sELISA, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CCL2.

P13500

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대체품

Primary Antibodies

AB214819

Anti-MCP1 antibody [EPR21025]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry - Anti-MCP1 antibody [EPR21025] (AB214819)

  • 0
  • 0
Primary Antibodies

AB242013

Anti-MCP1 antibody [EPR21025] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry - Anti-MCP1 antibody [EPR21025] - BSA and Azide free (AB242013)

  • 0
  • 0
Primary Antibodies

AB246795

Anti-MCP1 antibody [EPR21025] - Low endotoxin, Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Flow Cytometry - Anti-MCP1 antibody [EPR21025] - Low endotoxin, Azide free (AB246795)

  • 0
  • 0

보완 제품

Assay Kits

AB179886

Human MCP-1 ELISA Kit

Recombinant|SimpleStep

ELISA - Human MCP-1 ELISA Kit (AB179886)

  • 0
  • 0
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB182422

Anti-CD163 antibody [EPR19518]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Flow Cytometry - Anti-CD163 antibody [EPR19518] (AB182422)

  • 5
  • 17
Primary Antibodies

AB312914

Alexa Fluor® 594 Anti-CD11b antibody [M1/70]

Recombinant

Flow Cytometry - Alexa Fluor® 594 Anti-CD11b antibody [M1/70] (AB312914)

  • 0
  • 0
Primary Antibodies

AB281583

Anti-CD31 antibody [RM1006]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD31 antibody [RM1006] (AB281583)

  • 5
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "sELISA-species-checked": "predicted", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "1 µg/mL", "sELISA-species-notes": "<p>For sandwich ELISA, use this antibody as Capture at 1μg/ml with <a href='/ko/products/primary-antibodies/mcp1-antibody-ab9669'>ab9669</a> as Detection.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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제품 세부 정보

This product is no longer batch tested in IHC, for an IHC validated antibody please see ab73680
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특성 및 보관 정보

제형
Lyophilized
Reconstitution
Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Purification 테크닉
Affinity purification Protein A
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MCP-1 also known as CCL2 is a chemokine involved in the recruitment of monocytes to sites of injury and inflammation. This protein consists of 76 amino acids and has a molecular weight of about 11 kDa. MCP-1 is expressed in a wide range of cell types including endothelial cells fibroblasts and monocytes. It acts by binding to the chemokine receptor CCR2 stimulating intracellular signaling pathways that mediate cell migration.
Biological function summary

The role of MCP-1 is significant in guiding immune cells to areas requiring immune response. It does not form part of a complex but operates as a singular entity to affect immune signaling. MCP-1 influences the behavior of monocytes and macrophages by inducing chemotaxis and activating their effector functions. Through this mechanism it aids in the inflammatory response and tissue repair.

Pathways

MCP-1 participates in the inflammatory pathways by cooperating with key proteins like CCR2. It facilitates the JAK-STAT signaling pathway which is vital for transmitting extracellular information into cellular responses. The interaction of MCP-1 with CCR2 influences the activation of downstream proteins like STAT3 enabling various immune responses. MCP-1 thereby plays a central role in modulating inflammation-related pathways.

MCP-1 has a connection to inflammatory bowel disease and atherosclerosis. In such conditions elevated levels of MCP-1 can exacerbate inflammatory responses by recruiting monocytes to dysfunctional sites worsening tissue damage. Through its interaction with CCR2 MCP-1 becomes a pivotal factor in chronic inflammation. Targeting this MCP-1/CCR2 axis can provide therapeutic opportunities for modulating disease progression in these inflammatory diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Acts as a ligand for C-C chemokine receptor CCR2 (PubMed : 10529171, PubMed : 10587439, PubMed : 9837883). Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions (PubMed : 10587439, PubMed : 9837883). Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils (PubMed : 8195247, PubMed : 8627182, PubMed : 9792674). May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis (PubMed : 8107690).
See full target information CCL2

대체 명칭 보기

MCP1, SCYA2, CCL2, C-C motif chemokine 2, HC11, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, Small-inducible cytokine A2, MCAF, MCP-1
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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cardiovascular diabetology 23:444 PubMed39696214

2024

Pericoronary adipose tissue radiomics features as imaging markers for coronary artery disease risk assessment: insights from gene expression analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Weimin Zhang,Yahui Hu,Zheng Xu,Qiang Huo,Haicheng Qi,Qian Liu,Yan Xing

Heliyon 9:e17366 PubMed37426804

2023

circSnd1 promotes atherosclerosis progression through the miR-485-3p/Olr1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Yang,Yuhao Lin,Chao Wang,Pengcheng Fan

Diagnostics (Basel, Switzerland) 11: PubMed34067481

2021

Elevation of CD40/CD40L Inflammatory Pathway Molecules in Carotid Plaques from Moderate-and-Severe Obstructive Sleep Apnea Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Ewa Migacz,Wioletta Olejarz,Alicja Głuszko,Katarzyna Bednarek-Rajewska,Robert Proczka,David F Smith,Stacey L Ishman,Wojciech Kukwa

Oncology letters 15:7760-7768 PubMed29725470

2018

Mutant monocyte chemoattractant protein-1 protein (7ND) inhibits osteoclast differentiation and reduces oral squamous carcinoma cell bone invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Shuyu Luo,Chuanxiang Zhou,Jianming Zhang,Mengshan Chen,Hongjie Li,Shanchuan Zheng,Jingjing Quan

PloS one 9:e88952 PubMed24586454

2014

Monocyte chemotactic protein 1 (MCP-1) modulates pro-survival signaling to promote progression of head and neck squamous cell carcinoma.

Applications

IHC-P

Species

Human

Wen-Tsai Ji,Hau-Ren Chen,Chun-Hsuan Lin,Jeng-Woei Lee,Ching-Chih Lee

Frontiers in aging neuroscience 5:19 PubMed23675346

2013

Thrombin, a mediator of cerebrovascular inflammation in AD and hypoxia.

Applications

IHC-P

Species

Mouse

Debjani Tripathy,Alma Sanchez,Xiangling Yin,Jinhua Luo,Joseph Martinez,Paula Grammas
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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