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AB121277

Anti-MCPH1 antibody

4

(1 리뷰)

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(3 제품이 사용된 논문 )

Rabbit Polyclonal MCPH1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MCPH1 aa 550-750.

대체 명칭 보기

Microcephalin, MCPH1

5 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Immunohistochemical analysis of paraffin embedded human testis tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Immunohistochemical analysis of paraffin embedded human cerebellum tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Immunohistochemical analysis of paraffin embedded human liver tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCPH1 antibody (AB121277)

Immunohistochemical analysis of paraffin embedded human cerebral cortex tissue labeling MCPH1 with ab121277 antibody at a 1/50 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-MCPH1 antibody (AB121277)
  • WB

Supplier Data

Western blot - Anti-MCPH1 antibody (AB121277)

All lanes:

Western blot - Anti-MCPH1 antibody (ab121277) at 0.4 µg/mL

All lanes:

NTERA-2 (human malignant pluripotent embryonic carcinoma cell line) cell lysate

Predicted band size: 93 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MCPH1 aa 550-750. The exact immunogen used to generate this antibody is proprietary information.

Q8NEM0

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보완 제품

Primary Antibodies

AB8152

Anti-BrdU antibody [IIB5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [IIB5] (AB8152)

  • 5
  • 7
Primary Antibodies

AB80

Anti-CDKN2A/p19ARF antibody

Western blot - Anti-CDKN2A/p19ARF antibody (AB80)

  • 4
  • 15
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein Microcephalin 1 (MCPH1) also known as BRIT1 plays an important role in cellular processes. It has a molecular mass of approximately 104 kDa. MCPH1 is widely expressed in various tissues with notable expression in the brain and gonads. Its primary mechanical function involves DNA damage response where it helps maintain genome integrity by recruiting repair proteins to sites of damage.
Biological function summary

MCPH1 acts as an important regulator in cell cycle checkpoints particularly the G2/M checkpoint which ensures that cells do not undergo mitosis with damaged DNA. This protein can form complexes with several others to effectively control the replication and repair processes. MCPH1 positively influences chromatin remodeling aiding in both the condensation and decondensation of chromatin structures during different phases of the cell cycle.

Pathways

MCPH1 integrates into both the ATM/ATR and CHK1/CHK2 pathways which are important in DNA damage signaling. These pathways involve the ATM protein which is related to MCPH1 through DNA damage response. In these pathways MCPH1’s regulatory actions are critical for proper AT response and checkpoint activation demonstrating its significance in maintaining genomic stability and cellular function.

MCPH1 mutations have been linked to microcephaly a developmental disorder characterized by reduced brain size. Additionally research associates MCPH1 dysfunction with cancer progression due to its role in maintaining chromosomal stability. The protein p53 connects to MCPH1 through both microcephaly and cancer pathways as it acts in concert with MCPH1 to promote accurate DNA repair and cell cycle regulation.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.
See full target information MCPH1
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Medical science monitor : international medical jo 24:7451-7458 PubMed30337515

2018

Silencing BRIT1 Facilitates the Abilities of Invasiveness and Migration in Trophoblast Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Luping Liu,Li Sun,Jing Zheng,Yanchun Wang

PloS one 10:e0144957 PubMed26683461

2015

The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmin Ge,Lixiao Che,Chunying Du

Proceedings of the National Academy of Sciences of the United States of America 112:E1210-9 PubMed25733871

2015

BRUCE regulates DNA double-strand break response by promoting USP8 deubiquitination of BRIT1.

Applications

Unspecified application

Species

Unspecified reactive species

Chunmin Ge,Lixiao Che,Jinyu Ren,Raj K Pandita,Jing Lu,Kaiyi Li,Tej K Pandita,Chunying Du
제품이 사용된 논문 모두 보기
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Product promise

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