AB96193

Anti-MDH2 antibody

Name: Anti-MDH2 antibody
Brand: Abcam Limited
SKU: AB96193
Rating: 5 (2 reviews)

(2 리뷰들)

(11 제품이 사용된 논문 )

Rabbit Polyclonal MDH2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MDH2 aa 1-300.

6 이미지

Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody (AB96193)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody (AB96193)

Immunofluorescence staining of ice-cold methanol-fixed (5 minutes) HeLa cells stained for MDH2 with ab96193 at 1 : 500 dilution. Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (AB96193)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (AB96193)

Immunohistochemical analysis of paraffin embedded H1299 xenograft tissue labelling MDH2 with ab96193 antibody at 1/500 dilution.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (AB96193)

Immunohistochemical analysis of paraffin embedded mouse heart tissue labelling MDH2 with ab96193 antibody at 1/500 dilution at the mitochondria.

Supplier Data

Western blot - Anti-MDH2 antibody (AB96193)

10% SDS-PAGE

All lanes:

Western blot - Anti-MDH2 antibody (ab96193) at 1/5000 dilution

All lanes:

Rat lung at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 36 kDa

false

Supplier Data

Western blot - Anti-MDH2 antibody (AB96193)

10% SDS PAGE

All lanes:

Western blot - Anti-MDH2 antibody (ab96193) at 1/10000 dilution

All lanes:

Mouse brain at 20 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 36 kDa

false

AbReview23095****

Western blot - Anti-MDH2 antibody (AB96193)

All lanes:

Western blot - Anti-MDH2 antibody (ab96193) at 1/1000 dilution

Lane 1:

Mouse C2C12 whole cell lysate at 20 µg

Lane 2:

Mouse C2C12 whole cell lysate on day 1 of differentiation at 20 µg

Lane 3:

Mouse C2C12 whole cell lysate on day 2 of differentiation at 20 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG at 1/100000 dilution

Predicted band size: 36 kDa

Observed band size: 46 kDa

true

Exposure time: 30s

This image is courtesy of an Anonymous Abreview.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MDH2 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P40926

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "EDTA based, pH 8.0 buffer, 15min", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "EDTA based, pH 8.0 buffer, 15min", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MDH2 also known as malate dehydrogenase 2 or mitochondrial malate dehydrogenase is an enzyme with a molecular mass of approximately 35 kDa. This enzyme catalyzes the conversion of malate to oxaloacetate using NAD+ as a cofactor. It is predominantly expressed in the mitochondria where it plays an important role in cellular respiration. The enzyme enables the malate dehydrogenase reaction which is key for the functioning of the tricarboxylic acid cycle.

Biological function summary

MDH2 participates in the critical process of energy production within the cell. While it does not form a complex itself its activity is intimately connected with other enzymes in mitochondrial energy metabolism. The malate dehydrogenase assay often measures the activity of MDH2 to understand the metabolic status of cells. By facilitating the oxidation of malate MDH2 aids in maintaining the efficiency of the mitochondrial electron transport chain by regenerating NADH.

Pathways

The enzyme is essential in the tricarboxylic acid (TCA) cycle and the malate-aspartate shuttle. In the TCA cycle MDH2 collaborates with enzymes like citrate synthase and isocitrate dehydrogenase to assist in the conversion of acetyl-CoA into energy-rich molecules. The malate-aspartate shuttle on the other hand involves MDH2 working closely with aspartate transaminase to transfer reducing equivalents into the mitochondria. These pathways highlight MDH2’s importance in cellular energy homeostasis.

Mutations or dysregulation in MDH2 have connections to certain metabolic conditions and cancers. For example alterations in MDH2 activity might contribute to conditions like mitochondrial myopathy altering energy metabolism. Moreover MDH2 is associated with NADH-producing enzymes whose dysregulation can support oncogenic pathways in cancer. Understanding these associations helps researchers pursue therapeutic targets that modulate MDH2 activity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information MDH2

대체 명칭 보기

MDH2

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제품이 사용된 논문 (11)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JACS Au 5:302-319 PubMed39886595

2025

SMAP3-ID for Identification of Endogenous Protein-Protein Interactions Reveals Regulation of Mitochondrial Activity by Lamins.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Warren,Jian Wang,Francis Dhoro,Bo Chao,Ashok Reddy,Stefanie Kaech Petrie,Larry L David,Xiangshu Xiao,Bingbing X Li

Aging and disease 13:1532-1545 PubMed36186135

2022

Dietary Salt Disrupts Tricarboxylic Acid Cycle and Induces Tau Hyperphosphorylation and Synapse Dysfunction during Aging.

Applications

Unspecified application

Species

Unspecified reactive species

Minghao Yuan,Yangyang Wang,Jie Wen,Feng Jing,Qian Zou,Yinshuang Pu,Tingyu Pan,Zhiyou Cai

Cell reports 40:111105 PubMed35905715

2022

Skeletal progenitors preserve proliferation and self-renewal upon inhibition of mitochondrial respiration by rerouting the TCA cycle.

Applications

Unspecified application

Species

Unspecified reactive species

Guillaume Tournaire,Shauni Loopmans,Steve Stegen,Gianmarco Rinaldi,Guy Eelen,Sophie Torrekens,Karen Moermans,Peter Carmeliet,Bart Ghesquière,Bernard Thienpont,Sarah-Maria Fendt,Nick van Gastel,Geert Carmeliet

Cell metabolism 33:2464-2483.e18 PubMed34800366

2021

Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.

Applications

Unspecified application

Species

Unspecified reactive species

Marcel Morgenstern,Christian D Peikert,Philipp Lübbert,Ida Suppanz,Cinzia Klemm,Oliver Alka,Conny Steiert,Nataliia Naumenko,Alexander Schendzielorz,Laura Melchionda,Wignand W D Mühlhäuser,Bettina Knapp,Jakob D Busch,Sebastian B Stiller,Stefan Dannenmaier,Caroline Lindau,Mariya Licheva,Christopher Eickhorst,Riccardo Galbusera,Ralf M Zerbes,Michael T Ryan,Claudine Kraft,Vera Kozjak-Pavlovic,Friedel Drepper,Sven Dennerlein,Silke Oeljeklaus,Nikolaus Pfanner,Nils Wiedemann,Bettina Warscheid

Disease models & mechanisms 13: PubMed32928875

2020

Teleological role of L-2-hydroxyglutarate dehydrogenase in the kidney.

Applications

Unspecified application

Species

Unspecified reactive species

Garrett Brinkley,Hyeyoung Nam,Eunhee Shim,Richard Kirkman,Anirban Kundu,Suman Karki,Yasaman Heidarian,Jason M Tennessen,Juan Liu,Jason W Locasale,Tao Guo,Shi Wei,Jennifer Gordetsky,Teresa L Johnson-Pais,Devin Absher,Dinesh Rakheja,Anil K Challa,Sunil Sudarshan

Molecular genetics and metabolism 128:444-451 PubMed31648943

2019

Tricarboxylic acid cycle enzyme activities in a mouse model of methylmalonic aciduria.

Applications

Unspecified application

Species

Unspecified reactive species

Parith Wongkittichote,Gary Cunningham,Marshall L Summar,Elena Pumbo,Patrick Forny,Matthias R Baumgartner,Kimberly A Chapman

Clinical cancer research : an official journal of the American Association for Cancer Research 24:6433-6446 PubMed30108105

2018

Biochemical and Epigenetic Insights into L-2-Hydroxyglutarate, a Potential Therapeutic Target in Renal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sandeep Shelar,Eun-Hee Shim,Garrett J Brinkley,Anirban Kundu,Francesca Carobbio,Tyler Poston,Jubilee Tan,Vishwas Parekh,Daniel Benson,David K Crossman,Phillip J Buckhaults,Dinesh Rakheja,Richard Kirkman,Yusuke Sato,Seishi Ogawa,Shilpa Dutta,Sadanandan E Velu,Ethan Emberley,Alison Pan,Jason Chen,Tony Huang,Devin Absher,Anja Becker,Conrad Kunick,Sunil Sudarshan

Cell 169:1051-1065.e18 PubMed28575669

2017

The Mammalian Ribo-interactome Reveals Ribosome Functional Diversity and Heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Deniz Simsek,Gerald C Tiu,Ryan A Flynn,Gun W Byeon,Kathrin Leppek,Adele F Xu,Howard Y Chang,Maria Barna

Nature chemical biology 13:494-500 PubMed28263965

2017

L-2-Hydroxyglutarate production arises from noncanonical enzyme function at acidic pH.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew M Intlekofer,Bo Wang,Hui Liu,Hardik Shah,Carlos Carmona-Fontaine,Ariën S Rustenburg,Salah Salah,M R Gunner,John D Chodera,Justin R Cross,Craig B Thompson

PloS one 11:e0160384 PubMed27611087

2016

Novel Metabolic Abnormalities in the Tricarboxylic Acid Cycle in Peripheral Cells From Huntington's Disease Patients.

Applications

Species

Unspecified reactive species

Nima N Naseri,Joseph Bonica,Hui Xu,Larry C Park,Jamshid Arjomand,Zhengming Chen,Gary E Gibson

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MDH2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody (AB96193)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (AB96193)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (AB96193)

Western blot - Anti-MDH2 antibody (AB96193)

Western blot - Anti-MDH2 antibody (AB96193)

Western blot - Anti-MDH2 antibody (AB96193)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (11)

Product promise