AB223761

Anti-ME1 antibody

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Rabbit Polyclonal ME1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ME1 aa 250-450.

대체 명칭 보기

NADP-dependent malic enzyme, NADP-ME, Malic enzyme 1, ME1

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME1 antibody (AB223761)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME1 antibody (AB223761)

Paraffin-embedded human adrenal gland tissue stained for ME1 using ab223761 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ME1 antibody (AB223761)

All lanes:

Western blot - Anti-ME1 antibody (ab223761) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG (human brain glioma cell line) cell lysate

Lane 3:

Human plasma (IgG/HSA depleted)

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 64 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ME1 aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

P48163

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

ME1 also known as malic enzyme 1 is a cytosolic enzyme that catalyzes the reversible oxidative decarboxylation of malate to pyruvate generating NADPH in the process. This enzyme contributes to cellular lipid and cholesterol biosynthesis. ME1 has an approximate molecular weight of 64 kDa. You can find ME1 expression mainly in tissues with high biosynthetic activities such as liver fat and kidney.

Biological function summary

ME1 plays an important role in regulating the cellular redox state and maintaining metabolic homeostasis. It supplies NADPH which is necessary for fatty acid synthesis and lipid metabolism supporting anabolic processes in cells. ME1 works as a part of a metabolic enzyme complex that coordinates with other enzymes to produce reducing equivalents required for detoxification and biosynthesis. Scientists frequently study its role in lipid synthesis due to its implications in metabolic disorders.

Pathways

ME1 is involved in lipid metabolism and the pentose phosphate pathway where it helps generate reducing power. ME1 interacts with other enzymes like glucose-6-phosphate dehydrogenase which also provides NADPH showing its interconnected role in maintaining cellular redox balance. ME1’s activity supports both pathways by contributing to the production of ribose-5-phosphate and other precursors important for nucleotide biosynthesis and antioxidant defense.

ME1 has links to obesity and cancer where its dysregulation can promote tumorigenesis and metabolic disorders. Studies suggest aberrant ME1 expression may lead to altered lipid biosynthesis contributing to excessive fat accumulation in obesity. ME1 and glutamine synthetase a protein connected in cancer metabolism work together to support cell proliferation through their metabolic pathways highlighting ME1’s potential as a target in metabolic and cancer therapy.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP(+) and divalent metal ions, and decarboxylation of oxaloacetate.

See full target information ME1

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 13:2179 PubMed35449133

2022

Golgi apparatus-targeted aggregation-induced emission luminogens for effective cancer photodynamic therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Minglun Liu,Yuncong Chen,Yan Guo,Hao Yuan,Tongxiao Cui,Shankun Yao,Suxing Jin,Huanhuan Fan,Chengjun Wang,Ran Xie,Weijiang He,Zijian Guo

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ME1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME1 antibody (AB223761)

Western blot - Anti-ME1 antibody (AB223761)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise