Anti-ME2 antibody [EP7217]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EP7217] (AB139686)

Immunohistochemical analysis of paraffin-embedded Human papillary adenocarcinoma of thyroid tissue labeling ME2 with ab139686 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EP7217] (AB139686)

Immunohistochemical analysis of paraffin embedded Normal Human Tonsil tissue using ab139686 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EP7217] (AB139686)

Immunohistochemical analysis of paraffin embedded Normal Human Colon tissue using ab139686 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EP7217] (AB139686)

Immunohistochemical analysis of paraffin embedded Normal Human Brain tissue using ab139686 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EP7217] (AB139686)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling ME2 with ab139686 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ME2 antibody [EP7217] (AB139686)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab139686 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ME2 antibody [EP7217] (AB139686)

Overlay histogram showing HeLa cells stained with ab139686 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139686, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• WB

Unknown

Western blot - Anti-ME2 antibody [EP7217] (AB139686)

All lanes:

Western blot - Anti-ME2 antibody [EP7217] (ab139686) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HL60 cell lysate at 10 µg

Lane 4:

293T cell lysate at 10 µg

Lane 5:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 65 kDa

false