Anti-MED12 Antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human placenta using ab327176 at 1 : 20–1 : 50 dilution, showing high expression.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human cerebral cortex, colon, spleen and testis using ab327176 at 1 : 20–1 : 50 dilution (A) shows similar protein distribution across tissues to an independent Anti-MED12 antibody (B).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human spleen using ab327176 at 1 : 20–1 : 50 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemistry analysis in human placenta and pancreas tissues using ab327176 at 1 : 20–1 : 50 dilution. Corresponding MED12 RNA-seq data are presented for the same tissues.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human cerebral cortex using ab327176 at 1 : 20–1 : 50 dilution.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human colon using ab327176 at 1 : 20–1 : 50 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human pancreas using ab327176 at 1 : 20–1 : 50 dilution, showing low expression as expected.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MED12 Antibody (AB327176)

Immunohistochemical staining of human testis using ab327176 at 1 : 20–1 : 50 dilution.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-MED12 Antibody (AB327176)

Immunofluorescent staining of human cell line U-2 OS using ab327176 at 0.25–2 µg/ml, showing localization to nucleoplasm.

• ChIP

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ChIP - Anti-MED12 Antibody (AB327176)

ChIP-Exo-Seq composite graph for ab327176 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

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Western blot - Anti-MED12 Antibody (AB327176)

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using ab327176 at 0.04–0.4 µg/ml. Remaining relative intensity is presented.

All lanes:

Western blot - Anti-MED12 Antibody (ab327176)

Lane 2:

U2OS cells transfected with control siRNA

Lane 3:

U2OS cells transfected with target specific siRNA probe #1

Lane 4:

U2OS cells transfected with target specific siRNA probe #2

Predicted band size: 243.08 kDa

false