AB242058

Anti-MEF2B antibody [EPR22193-26]

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal MEF2B antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.

대체 명칭 보기

XMEF2, MEF2B, Myocyte-specific enhancer factor 2B, RSRFR2, Serum response factor-like protein 2

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Flow Cytometry (Intracellular) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized Daudi (human Burkitt's lymphoma cell line) cells labeling [MEF2B with ab242058 at 1/500 (red) compared with a rabbit monoclonal IgG (ab172730, black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on germinal center B cells of human tonsil (PMID : 23974956; PMID : 26089142) (PMID : 26089142) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Immunohistochemical analysis of paraffin-embedded human diffuse large B cell lymphoma tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on human diffuse large B-cell lymphoma cells (PMID : 26089142) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling MEF2B with ab242058 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on a subset of smooth muscle cells (arrowhead) of human colon (PMID : 26089142; PMID : 8668199) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Supplier Data

Immunoprecipitation - Anti-MEF2B antibody [EPR22193-26] (AB242058)

MEF2B was immunoprecipitated from 0.35 mg of Ramos (human Burkitt's lymphoma cell line) whole cell lysate with ab242058 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab242058 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Ramos whole lysate 10 μg (Input).
Lane 2 : ab242058 IP in Ramos whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab242058 in Ramos whole cell lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-MEF2B antibody [EPR22193-26] (ab242058)

Predicted band size: 39 kDa

false

Supplier Data

Western blot - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Blocking and dilution buffer : 5% NFDM/TBST.

The 40-kDa target band is consistent with what has been described in the literature (PMID : 23974956). Minor bands below 40kDa are undetermined proteins, likely to be truncated MEF2B (PMID : 23974956).

Negative control : Hela (PMID : 8798771).

All lanes:

Western blot - Anti-MEF2B antibody [EPR22193-26] (ab242058) at 1/1000 dilution

Lane 1:

Daudi (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 2:

Ramos (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 3:

Raji (human Burkitt's lymphoma cell line) whole cell lysate at 10 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 39 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22193-26

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MEF2B also known as myocyte enhancer factor 2B plays an integral role in regulating gene expression. It is a transcription factor belonging to the MEF2 family which also includes MEF2A MEF2C and MEF2D. MEF2B has a molecular mass of approximately 50 kilodaltons. Researchers have found its expression mainly in lymphoid tissues but it is also present in other tissue types. Through its actions MEF2B influences various cellular processes by binding to DNA and facilitating transcriptional activation.

Biological function summary

MEF2B functions as a regulator of immune system development and differentiation. It acts at the level of transcription to determine the fate of cells by its interactions. MEF2B often partners with various cofactors to form a complex which enhances its ability to control gene expression. It is known to be highly involved in muscle development and cell proliferation underlining its diverse roles in cellular biology.

Pathways

MEF2B plays important roles in two key signaling networks: the MAPK signaling pathway and the calcium signaling pathway. These pathways manage diverse biological processes like cell growth apoptosis and differentiation. Through the MAPK pathway MEF2B interacts with proteins such as ERK leading to various cellular outcomes. In the calcium signaling pathway MEF2B works alongside calcineurin a phosphatase important for activating MEF2 family members.

MEF2B has been linked with specific health conditions particularly in lymphoma and leukemia. Mutations and dysregulation in MEF2B may contribute to the development of these cancers. MEF2B's interaction with other proteins like BCL6 a well-known repressor in lymphomagenesis highlights its importance in these contexts. Understanding MEF2B's role in these diseases may provide insights for potential therapeutic strategies.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Activates transcription via this element. May be involved in muscle-specific and/or growth factor-related transcription.

See full target information MEF2B

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEF2B antibody [EPR22193-26]

Flow Cytometry (Intracellular) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Immunoprecipitation - Anti-MEF2B antibody [EPR22193-26] (AB242058)

Western blot - Anti-MEF2B antibody [EPR22193-26] (AB242058)

관련 conjugated 항체와 다양한 조성의 항체 (2)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise