AB78888

Anti-MEF2C (phospho S396) antibody

Name: Anti-MEF2C (phospho S396) antibody
Brand: Abcam Limited
SKU: AB78888
Rating: 4 (8 reviews)

(8 리뷰들)

(7 제품이 사용된 논문 )

Rabbit Polyclonal MEF2C phospho S396 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human MEF2C pS396.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C (phospho S396) antibody (AB78888)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C (phospho S396) antibody (AB78888)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using ab78888 at 1/50 dilution in the absence or presence of the immunzing phosphopeptide.

Unknown

Western blot - Anti-MEF2C (phospho S396) antibody (AB78888)

All lanes:

Western blot - Anti-MEF2C (phospho S396) antibody (ab78888) at 1/500 dilution

Lane 1:

Extracts from 24 hours starved 3T3 cells at 20 µg

Lane 2:

Extracts from 24 hours starved 3T3 cells at 20 µg with immunizing phosphopeptide

Predicted band size: 51 kDa

Observed band size: 51 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human MEF2C pS396. The exact immunogen used to generate this antibody is proprietary information.

Q06413

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Purified from rabbit antiserum by affinity chromatography, using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The MEF2C protein also known as MADS box transcription enhancer factor 2 polypeptide C plays an important role in gene expression regulation. This protein has a mass of approximately 52 kDa and is mainly found in the heart skeletal muscle brain and endothelial cells. It is part of the MEF2 family which includes other members like MEF2A MEF2B and MEF2D. MEF2C acts as a transcription factor binding to specific DNA sequences to activate the transcription of target genes involved in various biological processes.

Biological function summary

The MEF2C protein is essential in cellular differentiation and development. MEF2C is not part of a complex but interacts with other cofactors and regulatory proteins to fulfill its roles. It is important in the development of cardiac and skeletal muscle cells and plays a significant role in neurogenesis and neuronal connectivity in the central nervous system. This protein's precise regulation ensures proper development and function of these tissues and organs.

Pathways

MEF2C influences cellular signaling through the MAPK/ERK and calcium/calmodulin-dependent protein kinase pathways. In the MAPK/ERK pathway MEF2C modulates gene expression in response to external stimuli interacting with proteins like ERK1/2. In the calcium/calmodulin-dependent kinase pathway MEF2C plays a role in calcium signaling networks impacting neuronal plasticity and survival. These pathways highlight MEF2C’s involvement in muscle and brain function.

MEF2C mutations or dysregulation link to conditions like dilated cardiomyopathy and neurodevelopmental disorders such as MEF2C haploinsufficiency syndrome. In cardiac disease abnormal MEF2C activity can affect heart muscle cell function often involving proteins like actin and myosin. In neurodevelopmental disorders MEF2C's interaction with various transcriptional regulators impacts neuronal networks contributing to symptoms like impaired cognitive development and autism spectrum-like behaviors.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Enhances transcriptional activation mediated by SOX18. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoforms that lack the repressor domain are more active than isoform 1.

See full target information MEF2C pS396

대체 명칭 보기

Myocyte-specific enhancer factor 2C, Myocyte enhancer factor 2C, MEF2C

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Communications biology 8:310 PubMed40000807

2025

Mapk7 enhances osteogenesis and suppresses adipogenesis by activating Lrp6/β-catenin signaling axis in mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chuan Li,Jiahui Long,Shuqing Chen,Liru Tian,Ya Xiao,Shulin Chen,Deying Su,Baolin Zhang,Peiqiang Su,Liao Zhiheng,Caixia Xu

Genes & diseases 11:964-977 PubMed37692479

2023

deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling.

Applications

Species

Mouse

Chengzhi Wu,Hengyu Liu,Dongmei Zhong,Xiaoming Yang,Zhiheng Liao,Yuyu Chen,Shun Zhang,Deying Su,Baolin Zhang,Chuan Li,Liru Tian,Caixia Xu,Peiqiang Su

Molecules (Basel, Switzerland) 27: PubMed36014574

2022

MOBT Alleviates Pulmonary Fibrosis through an lncITPF-hnRNP-l-Complex-Mediated Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Pan Xu,Haitong Zhang,Huangting Li,Bo Liu,Rongrong Li,Jinjin Zhang,Xiaodong Song,Changjun Lv,Hongbo Li,Mingwei Chen

Disease models & mechanisms 15: PubMed34553752

2021

MEK-inhibitor-mediated rescue of skeletal myopathy caused by activating Hras mutation in a Costello syndrome mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

William E Tidyman,Alice F Goodwin,Yoshiko Maeda,Ophir D Klein,Katherine A Rauen

Developmental dynamics : an official publication of the American Association of Anatomists 248:795-812 PubMed31219639

2019

Cdc42 activation by endothelin regulates neural crest cell migration in the cardiac outflow tract.

Applications

Unspecified application

Species

Unspecified reactive species

Katrina R Fritz,Yanping Zhang,L Bruno Ruest

Journal of immunology (Baltimore, Md. : 1950) 201:2885-2898 PubMed30305329

2018

B Cell Development sans B Cell Receptor Responsiveness Due to Unfolded Protein Response-Triggered Mef2c Protein Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Ting Chen,Yu-Chia Su,John T Kung

Molecular medicine reports 13:2527-35 PubMed26848028

2016

Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells.

Applications

Species

Mouse

Bin Chen,Guixian Song,Ming Liu,Lingmei Qian,Lihua Wang,Haitao Gu,Yahui Shen

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEF2C (phospho S396) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEF2C (phospho S396) antibody (AB78888)

Western blot - Anti-MEF2C (phospho S396) antibody (AB78888)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (7)

Product promise