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AB265586

Anti-MEK2 antibody [BLR079G]

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal MEK2 antibody. Suitable for IHC-P, ICC, IP, WB, Flow Cyt and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human MAP2K2 aa 1-50.

대체 명칭 보기

MEK2, MKK2, PRKMK2, MAP2K2, Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2

6 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody [BLR079G] (AB265586)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody [BLR079G] (AB265586)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma tissue labeling MEK2 with ab265586 at 1/100 dilution. Detection : DAB.

Immunocytochemistry - Anti-MEK2 antibody [BLR079G] (AB265586)
  • ICC

Supplier Data

Immunocytochemistry - Anti-MEK2 antibody [BLR079G] (AB265586)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded KG-1 cells labeling MEK2 with ab265586 at 1/100 dilution. Detection : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody [BLR079G] (AB265586)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody [BLR079G] (AB265586)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling MEK2 with ab265586 at 1/100 dilution. Detection : DAB.

Flow Cytometry - Anti-MEK2 antibody [BLR079G] (AB265586)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-MEK2 antibody [BLR079G] (AB265586)

Flow cytometry overlay histogram of 4% formaldehyde fixed Jurkat cells permeabilized with 90% methanol labeling human MEK2 with ab265586 at 1 µl per 1 x 10^6 cells (shaded) compared with a Isotype control (unshaded). Secondary antibody details : DyLight® 488-conjugated goat anti-rabbit IgG (ab96883).

Immunoprecipitation - Anti-MEK2 antibody [BLR079G] (AB265586)
  • IP

Supplier Data

Immunoprecipitation - Anti-MEK2 antibody [BLR079G] (AB265586)

MEK2 was immunoprecipitated from 1mg of HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate with ab265586 at 20 μl/mg lysate. Western blot was performed from the immunoprecipitate using ab265586 at 1/1000 dilution.
Lane 1 : ab265586 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG.

Exposure time : 30 secs.

Cells prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-MEK2 antibody [BLR079G] (ab265586)

Predicted band size: 44 kDa

false

Western blot - Anti-MEK2 antibody [BLR079G] (AB265586)
  • WB

Supplier Data

Western blot - Anti-MEK2 antibody [BLR079G] (AB265586)

Cells prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-MEK2 antibody [BLR079G] (ab265586) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate at 50 µg

Lane 2:

HEK-293T whole cell lysate at 50 µg

Lane 3:

A549 whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

RKO whole cell lysate at 50 µg

Lane 6:

U-2 OS whole cell lysate at 50 µg

Lane 7:

LNCaP whole cell lysate at 50 µg

Lane 8:

HeLa whole cell lysate at 50 µg

Lane 9:

NIH/3T3 whole cell lysate at 50 µg

Lane 10:

TCMK whole cell lysate at 50 µg

Predicted band size: 44 kDa

false

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR079G

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, ICC, IP, Flow Cyt, WB

applications

Immunogen

Synthetic Peptide within Human MAP2K2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P36507

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대체품

Primary Antibodies

AB265601

Anti-MEK2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody (AB265601)

  • 0
  • 0
Primary Antibodies

AB214845

Alexa Fluor® 594 Anti-MEK2 antibody [Y78]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-MEK2 antibody [Y78] (AB214845)

  • 0
  • 0
Primary Antibodies

AB213661

PE Anti-MEK2 antibody [Y78]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-MEK2 antibody [Y78] (AB213661)

  • 0
  • 0
Primary Antibodies

AB32517

Anti-MEK2 antibody [Y78]

20ul selling size|KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MEK2 antibody [Y78] (AB32517)

  • 4
  • 3
Primary Antibodies

AB214633

Alexa Fluor® 555 Anti-MEK2 antibody [Y78]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-MEK2 antibody [Y78] (AB214633)

  • 0
  • 0
Primary Antibodies

AB200606

Alexa Fluor® 488 Anti-MEK2 antibody [Y78]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-MEK2 antibody [Y78] (AB200606)

  • 0
  • 0
Primary Antibodies

AB233731

Anti-MEK2 antibody [Y78] - BSA and Azide free

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-MEK2 antibody [Y78] - BSA and Azide free (AB233731)

  • 0
  • 0

보완 제품

Primary Antibodies

AB32537

Anti-ERK1 antibody [Y72]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-ERK1 antibody [Y72] (AB32537)

  • 5
  • 4
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p>Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with ICC staining protocol.</p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>20 μl per mg lysate.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µL for 10^6 Cells", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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제품 세부 정보

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
Purified from TCS.
보관 버퍼
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99% Borate buffered saline, 0.1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MEK2 or MAP2K2 is a protein kinase with a mass of approximately 44 kDa. This kinase part of the Mitogen-Activated Protein Kinase (MAPK) pathway primarily functions mechanically as a dual-specificity protein kinase. It phosphorylates both serine/threonine and tyrosine residues on its substrate proteins. MEK2 is largely expressed in various tissues including the brain and heart. It serves an important role in relaying extracellular signals within cells leading to diverse cellular responses.
Biological function summary

MEK2 participates in signal transduction pathways mediating cell division differentiation and apoptosis. As a part of the MAPK signaling cascade it operates downstream of RAS and RAF proteins. MEK2 forms a complex with MEK1 another closely related kinase to ensure proper signal relay. This complex plays a significant role in the regulation of cellular responses to growth signals and stress.

Pathways

MEK2 is intricately connected to the ERK1/2 pathway one of the key pathways it is involved in. ERK1/2 (Extracellular signal-Regulated Kinases) further transmits signals to the cell's nuclear components regulating gene expression critically. MEK2 directly phosphorylates and activates ERK1 and ERK2 facilitating their role in transcription cell cycle regulation and maintenance of cell integrity. The interaction of MEK2 with proteins like BRAF and CRAF along the MAPK pathway amplifies the signal transduction for various growth factors and hormones.

MEK2 has a notable association with cancer particularly melanoma and colorectal cancer. It often becomes aberrantly activated leading to unregulated cell proliferation. The connection of MEK2 to ERK1/2 proteins in these malignancies makes it a potential therapeutic target. Furthermore MEK2 mutations can contribute to Noonan syndrome a disorder that affects multiple body systems marking its relationship with genetic abnormalities and developmental issues. Researchers have been investigating MEK inhibitors to target these disorders highlighting the importance of understanding MEK2's role in disease contexts.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed : 29433126).
See full target information MAP2K2
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Anatomy & cell biology 56:526-537 PubMed37654017

2023

Effect of Sofosbuvir on rats' ovaries and the possible protective role of vitamin E: biochemical and immunohistochemical study.

Applications

Unspecified application

Species

Unspecified reactive species

Neven A Ebrahim,Hussein Abdelaziz Abdalla,Neimat Abd Elhakam Yassin,Aya Elsayed Maghrabia,Amira Ibrahim Morsy

Frontiers in molecular biosciences 8:729789 PubMed34621787

2021

Development of a Toll-Like Receptor-Based Gene Signature That Can Predict Prognosis, Tumor Microenvironment, and Chemotherapy Response for Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Liu,Bin Liu,Jie Yu,Dongyun Zhang,Jianhong Shi,Ping Liang

Experimental & molecular medicine 53:1356-1365 PubMed34531509

2021

MicroRNA-338-3p as a novel therapeutic target for intervertebral disc degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Jiang,Abu Moro,Jiaqi Wang,Dihua Meng,Xinli Zhan,Qingjun Wei
제품이 사용된 논문 모두 보기
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Product promise

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