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AB327588

Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free

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Rabbit Recombinant Monoclonal Melatonin Receptor 1A/MTNR1A antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate - Human, Mouse, Rat samples.
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Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)
  • WB

Supplier Data

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)

Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327588 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (ab327588) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extract (unboiled) at 30 µg

Lane 2:

HA-tagged MTNR1A transfected 293T whole cell extract (unboiled) at 30 µg

Lane 3:

DDDDK-tagged MTNR1B transfected 293T whole cell extract (unboiled) at 30 µg

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Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)
  • WB

Supplier Data

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)

Boiled and unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327588 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (ab327588) at 1/1000 dilution

Lane 1:

Rat eye tissue extract (boiled) at 1/50 dilution

Lane 2:

Rat liver tissue extract (boiled) at 1/50 dilution

Lane 3:

Rat eye tissue extract (unboiled) at 50 µg

Lane 4:

Rat liver tissue extract (unboiled) at 1/50 dilution

false

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)
  • WB

Supplier Data

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (AB327588)

Unboiled various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ab327588 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Melatonin Receptor 1A antibody [HL4732] - BSA and Azide free (ab327588) at 1/1000 dilution

Lane 1:

Mouse testis tissue extract (unboiled) at 50 µg

Lane 2:

Mouse eye tissue extract (unboiled) at 50 µg

Lane 3:

Mouse liver tissue extract (unboiled) at 50 µg

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL4732

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This antibody is specific for human MTNR1A protein and does not cross react with human MTNR1B protein.</p>

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

It was raised against human Melatonin Receptor 1A Extracellular domain.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
-20°C
적절한 장기 보관 조건
-20°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

High affinity receptor for melatonin. Likely to mediate the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Possibly involved in sleep induction, by melatonin activation of the potassium channel KCNMA1/BK and the dissociation of G-beta and G-gamma subunits, thereby decreasing synaptic transmission (By similarity).
See full target information Melatonin receptor type 1A

대체 명칭 보기

Melatonin receptor type 1A, Mel-1A-R, Mel1a receptor, MTNR1A
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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