Anti-Mesothelin antibody [EPR17823-69]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] (AB213174)

Immunohistochemical analysis of paraffin-embedded mouse spleen labelling Mesothelin with ab213174 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Positive staining on mesothelium of mouse spleen.

The section was incubated with ab213174 at 4°C overnight.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mesothelin antibody [EPR17823-69] (AB213174)

Immunohistochemical analysis of paraffin-embedded mouse lung labelling Mesothelin with ab213174 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Positive staining on mesothelium of mouse lung.

The section was incubated with ab213174 at 4°C overnight.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• IP

Supplier Data

Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] (AB213174)

Mesothelin was immunoprecipitated from 1 mg NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab213174 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab213174 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

Lane 1 : NIH/3T3 whole cell lysate 10 μg (Input).
Lane 2 : ab213174 IP NIH/3T3 whole cell lysate (+).
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab213174 in NIH/3T3 whole cell lysate (-)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-Mesothelin antibody [EPR17823-69] (ab213174)

Predicted band size: 69 kDa

Observed band size: 40 kDa,70 kDa

false

Exposure time: 3s

• WB

Supplier Data

Western blot - Anti-Mesothelin antibody [EPR17823-69] (AB213174)

Blocking and dilution buffer : 5% NFDM /TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 16857795; PMID : 17332303).

All lanes:

Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg

Lane 2:

Mouse lung lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 69 kDa

Observed band size: 40 kDa,71 kDa

true

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Mesothelin antibody [EPR17823-69] (AB213174)

Blocking and dilution buffer : 5% NFDM /TBST.

Lane 1:

Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/20000 dilution

Lane 2:

Western blot - Anti-Mesothelin antibody [EPR17823-69] (ab213174) at 1/100000 dilution

All lanes:

His-tagged mouse mesothelin fragment recombinant protein (aa298-600) 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

Observed band size: 40 kDa

true

Exposure time: 1min