AB242528

Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture) (ab242528) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Mesothelin in Western Blot, ELISA. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

2 이미지

Lab

Western blot - Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture) (AB242528)

This image was produced using ab196235, the same clone but in a different formulation.

False colour image of Western blot : Anti-Mesothelin antibody [EPR19025-42] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab196235 was shown to bind specifically to Mesothelin. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Mesothelin antibody [EPR19025-42] (<a href='/ko/products/primary-antibodies/mesothelin-antibody-epr19025-42-ab196235'>ab196235</a>) at 1/1000 dilution

Lane 1:

OVCAR-3 cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

PC-3 cell lysate at 20 µg

Predicted band size: 69 kDa

Observed band size: 45 kDa

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Sandwich ELISA - Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture) (AB242528)

sELISA

Supplier Data

Sandwich ELISA - Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture) (AB242528)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab216168).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19025-42

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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제품 세부 정보

ab242528 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Mesothelin. The recommended pair for sandwich ELISA is:
Capture: ab242528, Human Mesothelin Capture Antibody (unconjugated)
Detector: ab242775, Human Mesothelin Detector Antibody (unconjugated)
The reference range value is 15.62 - 1000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Membrane-anchored forms may play a role in cellular adhesion.. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

See full target information MSLN

대체 명칭 보기

MPF, MSLN, Mesothelin, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture)

Western blot - Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture) (AB242528)

Sandwich ELISA - Anti-Mesothelin antibody [EPR19025-42] - BSA and Azide free (Capture) (AB242528)

관련 conjugated 항체와 다양한 조성의 항체 (5)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Sample Prep & Detection Kits

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise