Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [RP23040220]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 Antibody [RP23040220] (AB313416)

Immunofluorescent analysis of HEK293 cells stably transfected with Metabotropic Glutamate Receptor 4/MGLUR4 fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer, then permeabilized with ice-cold methanol and then incubated with ab313416 at 1 µg/mL concentration, labeling Metabotropic Glutamate Receptor 4/MGLUR4 followed by Alexa Fluor® 488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope.

• WB

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 Antibody [RP23040220] (AB313416)

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 Antibody [RP23040220] (ab313416) at 1 µg/mL

All lanes:

HEK293 cells stably transfected with Metabotropic Glutamate Receptor 4/MGLUR4 were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads

Observed band size: 110 kDa,220 kDa

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