Anti-Methionyl Aminopeptidase 1/MAP 1 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody (AB241293)

Methionyl Aminopeptidase 1/MAP 1 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241293 used for IP at 20 μl per reaction. For WB at a 1 : 100 dilution.

Lane 1 : ab241293 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody (ab241293)

Predicted band size: 43 kDa

false

• WB

Supplier Data

Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody (AB241293)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody (ab241293) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 43 kDa

false

Exposure time: 30s