AB243074

Anti-Methylglyoxal antibody [9F11]

Name: Anti-Methylglyoxal antibody [9F11]
Brand: Abcam Limited
SKU: AB243074
Rating: 5 (2 reviews)

(2 리뷰들)

(10 제품이 사용된 논문 )

Mouse Monoclonal Methylglyoxal antibody. Suitable for WB, Flow Cyt and reacts with Chemical samples. Cited in 10 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Methylglyoxal.

대체 명칭 보기

2 oxo propanal, pyruvaldehyde

2 이미지

Flow Cytometry - Anti-Methylglyoxal antibody [9F11] (AB243074)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Methylglyoxal antibody [9F11] (AB243074)

Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (+ 250 μM hydrogen peroxide treatment for 24 hours) labeling Methylglyoxal with ab243074 at 1/50 dilution for 30 minutes on ice (solid line) compared with an Isotype control IgG (dashed line). A Goat Anti-Mouse : PE at 1/100 dilution was used as the secondary antibody (20 mins at RT).

Western blot - Anti-Methylglyoxal antibody [9F11] (AB243074)

Primary incubation for 2 hours at room temperature.

Secondary incubation for 1 hour at room temperature.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-Methylglyoxal antibody [9F11] (ab243074) at 1/1000 dilution

Lane 1:

Methylglyoxal-BSA at 0.5 µg

Lane 2:

BSA at 0.5 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

true

Exposure time: 5min

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

9F11

Isotype

IgG1

Carrier free

Applications

Flow Cyt, WB

applications

Specificity

Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, MDA, 4-HHE, 4-HNE, or Crotonaldehyde modified proteins.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from TCS.

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

See full target information Methylglyoxal

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제품이 사용된 논문 (10)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 25: PubMed39769037

2025

L. Leaf Extract Dose-Dependently Modulates Oxidative Stress in the Kidney and Exerts Anti-Fibrotic and Anti-Inflammatory Properties by the Molecular Mechanisms Independent of NRF-2 Signalization Mirroring the Effects of Losartan in SHR.

Applications

Unspecified application

Species

Unspecified reactive species

Una-Jovana Vajic,Nevena Mihailovic-Stanojevic,Danijela Karanovic,Maja Zivotic,Milan Ivanov,Djurdjica Jovovic,Jelica Grujic-Milanovic,Zoran Miloradovic

Molecules (Basel, Switzerland) 29: PubMed39519689

2024

Methylglyoxal-Induced Modifications in Human Triosephosphate Isomerase: Structural and Functional Repercussions of Specific Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Ignacio de la Mora-de la Mora,Itzhel García-Torres,Luis Antonio Flores-López,Gabriel López-Velázquez,Gloria Hernández-Alcántara,Saúl Gómez-Manzo,Sergio Enríquez-Flores

Redox biology 65:102843 PubMed37573838

2023

Methylglyoxal suppresses microglia inflammatory response through NRF2-IκBζ pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Li Wei,Ying Yang,Wei-Yue Si,Yang Zhou,Tao Li,Tong Du,Peng Zhang,Xiao-Li Li,Ruo-Nan Duan,Rui-Sheng Duan,Chun-Lin Yang

eLife 12: PubMed37548361

2023

DJ-1 paralogs maintain genome integrity through glycation repair of nucleic acids and proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Gautam Susarla,Priyanka Kataria,Amrita Kundu,Patrick D'Silva

International journal of molecular sciences 24: PubMed37298498

2023

Metformin Counteracts the Deleterious Effects of Methylglyoxal on Ovalbumin-Induced Airway Eosinophilic Inflammation and Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Matheus L Medeiros,Akila L Oliveira,Glaucia C Mello,Edson Antunes

Amino acids 55:413-420 PubMed36637533

2023

Carnosine increases insulin-stimulated glucose uptake and reduces methylglyoxal-modified proteins in type-2 diabetic human skeletal muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph J Matthews,Mark D Turner,Livia Santos,Kirsty J Elliott-Sale,Craig Sale

Journal of molecular and cellular cardiology 174:101-114 PubMed36481511

2022

Distinct effects of intracellular vs. extracellular acidic pH on the cardiac metabolome during ischemia and reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander S Milliken,Jessica H Ciesla,Sergiy M Nadtochiy,Paul S Brookes

The New phytologist 236:1061-1074 PubMed35976797

2022

Double DJ-1 domain containing Arabidopsis DJ-1D is a robust macromolecule deglycase.

Applications

Unspecified application

Species

Unspecified reactive species

Melvin Prasad,Priyanka Kataria,Sunayana Ningaraju,Radhika Buddidathi,Kondalarao Bankapalli,Chenna Swetha,Gautam Susarla,Radhika Venkatesan,Patrick D'Silva,Padubidri V Shivaprasad

eLife 9: PubMed32795389

2020

ALKBH7 mediates necrosis via rewiring of glyoxal metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Chaitanya A Kulkarni,Sergiy M Nadtochiy,Leslie Kennedy,Jimmy Zhang,Sophea Chhim,Hanan Alwaseem,Elizabeth Murphy,Dragony Fu,Paul S Brookes

Cancers 12: PubMed32466621

2020

Genetic Target Modulation Employing CRISPR/Cas9 Identifies Glyoxalase 1 as a Novel Molecular Determinant of Invasion and Metastasis in A375 Human Malignant Melanoma Cells In Vitro and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Jandova,Jessica Perer,Anh Hua,Jeremy A Snell,Georg T Wondrak

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Methylglyoxal antibody [9F11]

Flow Cytometry - Anti-Methylglyoxal antibody [9F11] (AB243074)

Western blot - Anti-Methylglyoxal antibody [9F11] (AB243074)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (10)

Product promise