AB220030

Anti-METTL14 antibody [CL4252]

Name: Anti-METTL14 antibody [CL4252]
Brand: Abcam Limited
SKU: AB220030
Rating: 3 (3 reviews)

(3 리뷰들)

(55 제품이 사용된 논문 )

Mouse Monoclonal METTL14 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 55 publications. Immunogen corresponding to Recombinant Fragment Protein within Human METTL14 aa 1-100.

대체 명칭 보기

KIAA1627, METTL14, N(6)-adenosine-methyltransferase non-catalytic subunit METTL14, Methyltransferase-like protein 14, hMETTL14

9 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cervix tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in squamous ephitelium and connective tissue cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testis tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in cells in seminiferous tubes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human prostate tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in glandular cells and in smooth muscle.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human endometrium tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear immunoreactivity in glandular cells and in stroma.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human duodenum tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in glandular cells and in the connective tissue.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cerebral cortex tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing moderate nuclear positivity in neurons.

Immunocytochemistry/ Immunofluorescence - Anti-METTL14 antibody [CL4252] (AB220030)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-METTL14 antibody [CL4252] (AB220030)

Immunocytochemistry/ Immunofluorescence anaysis of RH-30 cells labeling METTL14 with ab220030 at 2-10 μg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).

Supplier Data

Western blot - Anti-METTL14 antibody [CL4252] (AB220030)

All lanes:

Western blot - Anti-METTL14 antibody [CL4252] (ab220030) at 1/500 dilution

All lanes:

A431 cell line lysate

Predicted band size: 52 kDa

false

CiteAb

Western blot - Anti-METTL14 antibody [CL4252] (AB220030)

METTL14 western blot using anti-METTL14 antibody [CL4252] ab220030. Publication image and figure legend from Zhang, C., Zhang, M., et al., 2019, Cancer Med, PubMed 31243897.

ab220030 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab220030 please see the product overview.

Reduced m6A activated Wnt and PI3K‐Akt signaling in GC cells. After (A) METTL14 or (B) FTO knockdown, changes of major components in Wnt and PI3K‐Akt pathways were assessed by western blot. (C) A hypothetical outline of m6A's roles in GC progression through its interactions with upstream or downstream signaling networks. GC, gastric cancer

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL4252

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human METTL14 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9HCE5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

METTL14 also known in scientific circles as Methyltransferase-like 14 plays an important mechanical role in RNA methylation. This protein with a molecular weight of approximately 58 kDa functions as part of the m6A methyltransferase complex. METTL14 is mainly expressed in the nucleus of cells where it helps catalyze the formation of N6-methyladenosine (m6A) on RNA which is a significant epigenetic modification.

Biological function summary

METTL14 contributes greatly to the modification of messenger RNA (mRNA) through its involvement in the m6A methyltransferase complex alongside METTL3. This protein complex operates to methylate adenine residues in mRNA influencing mRNA stability splicing translation and eventually degradation. METTL14's function in this complex is more regulatory stabilizing the interaction between METTL3 and its RNA substrate modulating the final methylation outcomes.

Pathways

METTL14's methylation activity greatly impacts gene expression through the mRNA processing and metabolism pathways. The m6A modification where METTL14 acts is important in mRNA fate and participates significantly in pathways controlling cell differentiation and proliferation. Researchers link it to major proteins like YTH domain family proteins which recognize and bind to m6A-modified RNA propagating the downstream effects of mRNA processing.

Researchers have drawn significant links between METTL14 and various forms of cancer including leukemia. Altered METTL14 expression can dysregulate mRNA metabolism leading to tumorigenesis. Additionally METTL14 has been implicated in neurodevelopmental disorders due to its role in brain development and function. Variations in METTL14 expression or activity may influence these diseases through its interactions with METTL3 and other complex components that can affect gene expression patterns important for normal cell function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed : 24316715, PubMed : 24407421, PubMed : 25719671, PubMed : 27281194, PubMed : 27373337, PubMed : 29348140). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed : 27281194, PubMed : 27373337, PubMed : 27627798, PubMed : 29348140). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed : 24316715, PubMed : 24407421, PubMed : 25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis : m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).

See full target information METTL14

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제품이 사용된 논문 (55)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cellular & molecular biology letters 30:109 PubMed41039223

2025

RSL3 promotes PARP1 apoptotic functions by distinct mechanisms during ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dejian Chen,Fei Xie,Yimei Mo,Dan Qin,Binjiao Zheng,Lingyan Chen

Cell death discovery 11:397 PubMed40846737

2025

WTAP participates in the DNA damage response via an mA-FOXM1-dependent manner in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Huang,Zhixuan Bian,Chang Xu,Yue Zhang,Li Liu,Zhongqi Cui,Shasha Zhao,Qiangyuan Fan,Shaobo Xue,Yan Chen,Qiuhui Pan,Fenyong Sun

RSC medicinal chemistry 16:4194-4207 PubMed40599585

2025

Structure-guided design of a methyltransferase-like 3 (METTL3) proteolysis targeting chimera (PROTAC) incorporating an indole-nicotinamide chemotype.

Applications

Unspecified application

Species

Unspecified reactive species

Annabelle C Weldert,Ariane F Frey,Mackenzie W Krone,Franziska Krähe,Hannah Kuhn,Christian Kersten,Fabian Barthels

Nucleic acids research 53: PubMed40219966

2025

Poly-(ADP-ribose) serves as a scaffold for the methyltransferase METTL3/14 complex in the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Claudia Gonzalez-Leal,Jin Cai,Bram A F J de Groot,Andreas Wegerer,Julia Preisser,Martijn S Luijsterburg,Andreas G Ladurner

Clinical and experimental medicine 25:3 PubMed39527301

2024

Analysis of METTL14 expression in pancreatic cancer and adjacent tissues and its prognostic value for patient outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Siyu Meng,Cong Wang

The Journal of biological chemistry 300:107783 PubMed39303913

2024

Downregulation of the mA reader YTHDC2 upregulates exosome content in lung adenocarcinoma via inhibiting IFIT and OAS family members.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Yin,Lifang Ma,Xiaoting Tian,Qi Sun,Congcong Zhang,Yikun Wang,Yayou Miao,Xiangfei Xue,Yongjie Wang,Jiayi Wang,Xiao Zhang,Xumin Hou

Journal of orthopaedic translation 48:190-203 PubMed39280633

2024

METTL14-mediated HOXA5 mA modification alleviates osteoporosis via promoting WNK1 transcription to suppress NLRP3-dependent macrophage pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Tang,Yuxuan Du,Zejiu Tan,Dongpeng Li,Jiang Xie

Experimental and therapeutic medicine 28:375 PubMed39113907

2024

N6‑methyladenosine methyltransferase METTL14 is associated with macrophage polarization in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Ziheng Zhu,Lei Wan

The Journal of biological chemistry 300:107598 PubMed39059495

2024

N6-methyladenosine triggers renal fibrosis via enhancing translation and stability of ZEB2 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Yating Cai,Jiawang Zhou,Abai Xu,Jinchang Huang,Haisheng Zhang,Guoyou Xie,Ke Zhong,You Wu,Pengfei Ye,Hongsheng Wang,Hongxin Niu

World journal of surgical oncology 22:128 PubMed38725005

2024

METTL14 regulates CD8T-cell activation and immune responses to anti-PD-1 therapy in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chongqi Sun,Jian Wang,Huixing Li,Luyao Liu,Yu Lin,Ling Zhang,Xianglong Zu,Yizhi Zhu,Yongqian Shu,Dong Shen,Qiong Wang,Yiqian Liu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-METTL14 antibody [CL4252]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL14 antibody [CL4252] (AB220030)

Immunocytochemistry/ Immunofluorescence - Anti-METTL14 antibody [CL4252] (AB220030)

Western blot - Anti-METTL14 antibody [CL4252] (AB220030)

Western blot - Anti-METTL14 antibody [CL4252] (AB220030)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (55)

Product promise