AB97881

Anti-MIA3/TANGO1 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal MIA3/TANGO1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MIA3 aa 1-150.

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-MIA3/TANGO1 antibody (AB97881)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MIA3/TANGO1 antibody (AB97881)

Immunofluorescence analysis of methanol fixed HepG2 cells, using ab97881 at a 1/200 dilution.
Lower image : costained with Hoechst 33342.

Supplier Data

Western blot - Anti-MIA3/TANGO1 antibody (AB97881)

5% SDS PAGE.

All lanes:

Western blot - Anti-MIA3/TANGO1 antibody (ab97881) at 1/1000 dilution

Lane 1:

A549 whole cell lysate at 30 µg

Lane 2:

Hep G2 whole cell lysate at 30 µg

Predicted band size: 213 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MIA3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q5JRA6

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

MIA3/TANGO1 also known as Melanoma Inhibitory Activity 3 or Transport and Golgi Organization 1 is a protein with a molecular mass of approximately 190 kDa. This protein mainly expresses in the endoplasmic reticulum (ER). It plays an essential role in the secretory pathway by mediating the export of large molecules such as collagen from the ER to the Golgi apparatus. MIA3/TANGO1 acts as a scaffold organizing COPII vesicle coating components to facilitate the correct packaging and export of secretory proteins.

Biological function summary

MIA3/TANGO1 integrates into a larger ER export complex. This complex includes other proteins like cTAGE5 and Sec12 which assist in the proper assembly and budding of cargo-loaded vesicles. It maintains cellular homeostasis by ensuring cells correctly secrete ECM molecules such as collagen. Proper ECM molecule assembly and export are critical for maintaining tissue architecture particularly in connective tissues where collagen forms a substantial part of the structural matrix.

Pathways

MIA3/TANGO1 participates in the collagen biosynthesis and trafficking pathway. It actively interacts with proteins like cTAGE5 and Sec23 which are key components in the COPII vesicle formation. By influencing these pathways it affects the overall dynamics of the secretory machinery contributing to the accurate delivery of structural proteins necessary for cell structure integrity. MIA3/TANGO1's activity impacts the efficiency of the ER-to-Golgi transport an essential transition within the secretory pathway.

MIA3/TANGO1's dysfunction links to connective tissue disorders and certain forms of cancer. Mutations in MIA3/TANGO1 impact collagen secretion efficiency leading to conditions like Ehlers-Danlos syndrome a connective tissue disorder. Its interactions with proteins involved in these diseases such as HSP47 (a collagen-specific chaperone) further highlight its pathological relevance. Abnormal MIA3/TANGO1 function also associates with tumorigenesis as its role in ECM regulation could influence the tumor microenvironment impacting cancer progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a role in the transport of cargos that are too large to fit into COPII-coated vesicles and require specific mechanisms to be incorporated into membrane-bound carriers and exported from the endoplasmic reticulum. This protein is required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. It may participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. It is also specifically required for the secretion of lipoproteins by participating in their export from the endoplasmic reticulum (PubMed : 19269366, PubMed : 27138255). Required for correct assembly of COPII coat components at endoplasmic reticulum exit sites (ERES) and for the localization of SEC16A and membrane-bound ER-resident complexes consisting of MIA2 and PREB/SEC12 to ERES (PubMed : 28442536).

See full target information MIA3

대체 명칭 보기

KIAA0268, TANGO, UNQ6077/PRO20088, MIA3, Transport and Golgi organization protein 1 homolog, TANGO1, C219-reactive peptide, D320, Melanoma inhibitory activity protein 3

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of gastrointestinal oncology 12:52-68 PubMed33708424

2021

Exosomal miR-30a and miR-222 derived from colon cancer mesenchymal stem cells promote the tumorigenicity of colon cancer through targeting MIA3.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Du,Xuan Ye,Sheng-Rong Lu,Huan Li,Hong-Yue Liu,Qing Zhai,Bo Yu

PloS one 13:e0189711 PubMed29298329

2018

Molecular and functional analysis of anchorage independent, treatment-evasive neuroblastoma tumorspheres with enhanced malignant properties: A possible explanation for radio-therapy resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara J Abou-Antoun,Javad Nazarian,Anthony Ghanem,Stanislav Vukmanovic,Anthony D Sandler

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-MIA3/TANGO1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-MIA3/TANGO1 antibody (AB97881)

Western blot - Anti-MIA3/TANGO1 antibody (AB97881)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise