Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] - BSA and Azide free (AB324166)

This data was developed using ab324163, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Mouse CCL19 expression vector containing a His tag. (B) HEK-293T transfected with a Human CCL19 expression vector containing a His tag. (C) HEK-293T transfected with empty vector containing a His tag. tissue labeling MIP-3 beta/CCL19 with ab324163 at 1/5000 (0.102 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on HEK-293T transfected with a His-tagged Mouse CCL19 construct (image A), no staining on HEK-293T transfected with a His-tagged Human CCL19 construct (image B) and HEK-293T transfected with empty vector containing a His tag (image C) . The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] - BSA and Azide free (AB324166)

This data was developed using ab324163, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse thymus tissue labeling MIP-3 beta/CCL19 with ab324163 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse thymus. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] - BSA and Azide free (AB324166)

This data was developed using ab324163, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling MIP-3 beta/CCL19 with ab324163 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on mouse liver. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] - BSA and Azide free (AB324166)

This data was developed using ab324163, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse lymph node tissue labeling MIP-3 beta/CCL19 with ab324163 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse lymph node. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] - BSA and Azide free (AB324166)

This data was developed using ab324163, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling MIP-3 beta/CCL19 with ab324163 at 1/1000 (0.512 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse spleen. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Slides were incubated with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] - BSA and Azide free (AB324166)

This data was developed using ab324163, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : brain (PMID : 9653094)

The identity of the bands higher than 12kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (<a href='/ko/products/primary-antibodies/mip-3-beta-ccl19-antibody-epr27463-29-ab324163'>ab324163</a>) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 40 µg

Lane 2:

Mouse brain tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 12 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] - BSA and Azide free (AB324166)

This data was developed using ab324163, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands higher than 12kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-MIP-3 beta/CCL19 antibody [EPR27463-29] (<a href='/ko/products/primary-antibodies/mip-3-beta-ccl19-antibody-epr27463-29-ab324163'>ab324163</a>) at 1/1000 dilution

All lanes:

Mouse lymph node tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 12 kDa,36 kDa

false

Exposure time: 180s